Development of 14 microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus) using next generation sequencing technology.

IF 0.9 4区环境科学与生态学 Q4 BIODIVERSITY CONSERVATION

Conservation Genetics Resources Pub Date : 2014-06-01 DOI:10.1007/s12686-013-0115-2

Christina T Ruiz-Rodriguez, Yasuko Ishida, Alex D Greenwood, Alfred L Roca

引用次数: 16

Abstract

We report the development of 14 new microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus). Ten unrelated Queensland koala individuals from the San Diego Zoo, USA, were genotyped. The number of alleles per locus ranged from 2 to 7, with an average of 5.14 alleles per locus. Across all loci, the average observed and expected heterozygosity values were both 0.69. These polymorphic microsatellite loci will be useful for genetic studies relevant to the conservation of the koala, a species listed as vulnerable.

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利用下一代测序技术在昆士兰考拉(Phascolarctos cinereus adusstus)中开发14个微卫星标记。

本文报道了在昆士兰考拉(Phascolarctos cinereus adusus)中发现的14个新的微卫星标记。来自美国圣地亚哥动物园的10只无亲缘关系的昆士兰考拉进行了基因分型。每个基因座的等位基因数为2 ~ 7个，平均为5.14个。所有位点的平均观察杂合度和预期杂合度均为0.69。这些多态微卫星位点将有助于与保护被列为易危物种的考拉有关的遗传研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Conservation Genetics Resources BIODIVERSITY CONSERVATION-GENETICS & HEREDITY

CiteScore

2.90

自引率

9.10%

发文量

审稿时长

3-6 weeks

期刊介绍： Conservation Genetics Resources promotes the conservation of genetic diversity and advances the study of conservation genetics by providing rapid publication of technical papers and reviews on methodological innovations or improvements, computer programs, and genomic resources, as well as on the practical application of these resources towards the development of effective conservation policy and practice.