Identification of endoglin-dependent BMP-2-induced genes in the murine periodontal ligament cell line PDL-L2.

Q2 Biochemistry, Genetics and Molecular Biology

Journal of Molecular Signaling Pub Date : 2014-06-14 eCollection Date: 2014-01-01 DOI:10.1186/1750-2187-9-5

Osamu Ishibashi, Takashi Inui

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引用次数: 4

Abstract

Background: The periodontal ligament (PDL), connective tissue located between the cementum of teeth and alveolar bone of the mandibula, plays an important role in the maintenance and regeneration of periodontal tissues. We reported previously that endoglin was involved in the BMP-2-induced osteogenic differentiation of mouse PDL cells, which is associated with Smad-2 phosphorylation but not Smad-1/5/8 phosphorylation. In this study, to elucidate the detailed mechanism underlying the BMP-2 signalling pathway unique to PDL cells, we performed a microarray analysis to identify BMP-2-inducible genes in PDL-L2 cells, a mouse PDL-derived cell line, with or without endoglin knockdown.

Findings: Sixty-four genes were upregulated more than twofold by BMP-2 in PDL-L2 cells. Of these genes, 11 were endoglin-dependent, including Id4, which encodes ID4, a helix-loop-helix transcription factor closely associated with TGF-β signaling and osteoblast differentiation. The endoglin-dependent induction of ID4 by BMP-2 was also verified at a protein level.

Conclusion: Our findings indicate that ID4 could be a signal mediator involved in the BMP-2-induced endoglin-dependent osteogenic differentiation of PDL cells.

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小鼠牙周膜细胞系PDL-L2中内啡肽依赖性bmp -2诱导基因的鉴定。

背景:牙周韧带(periodontal ligament, PDL)是位于牙骨质和下颌骨牙槽骨之间的结缔组织，在牙周组织的维持和再生中起重要作用。我们之前报道过内啡肽参与bmp -2诱导的小鼠PDL细胞成骨分化，这与Smad-2磷酸化有关，而与Smad-1/5/8磷酸化无关。在这项研究中，为了阐明PDL细胞特有的BMP-2信号通路的详细机制，我们进行了微阵列分析，以鉴定PDL- l2细胞(一种小鼠PDL来源的细胞系)中BMP-2诱导基因，内啡肽敲低或不敲低。结果:64个基因在PDL-L2细胞中被BMP-2上调2倍以上。在这些基因中，有11个是内啡肽依赖的，包括Id4，它编码Id4，这是一种与TGF-β信号传导和成骨细胞分化密切相关的螺旋-环-螺旋转录因子。在蛋白水平上也证实了BMP-2对ID4的内啡肽依赖性诱导。结论:我们的研究结果表明，ID4可能是参与bmp -2诱导的内啡肽依赖性PDL细胞成骨分化的信号介质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Signaling Biochemistry, Genetics and Molecular Biology-Biochemistry

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期刊介绍： Journal of Molecular Signaling is an open access, peer-reviewed online journal that encompasses all aspects of molecular signaling. Molecular signaling is an exponentially growing field that encompasses different molecular aspects of cell signaling underlying normal and pathological conditions. Specifically, the research area of the journal is on the normal or aberrant molecular mechanisms involving receptors, G-proteins, kinases, phosphatases, and transcription factors in regulating cell proliferation, differentiation, apoptosis, and oncogenesis in mammalian cells. This area also covers the genetic and epigenetic changes that modulate the signaling properties of cells and the resultant physiological conditions.