SCCmec types and pvl gene in methicillin-resistant Staphylococcus aureus strains from children hospitalized in a tertiary care hospital in Mexico.

Scandinavian Journal of Infectious Diseases Pub Date : 2014-07-01 Epub Date: 2014-05-16 DOI:10.3109/00365548.2014.912349

Eunice Mireya Borbón-Esquer, Alberto Villaseñor-Sierra, Erika Martínez-López, Juan José Jáuregui-Lomeli, Rosa Villaseñor-Martínez, Mariana Del Rocío Ruiz-Briseño

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引用次数: 7

Abstract

Background: The aim of this study was to determine the prevalence, SCCmec types, presence of the Panton-Valentine leukocidin (PVL) gene, and susceptibility to antibiotics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from hospitalized children.

Methods: From August 2009 to September 2011, 291 S. aureus strains were isolated from normally sterile body sites, of which 190 (65%) were MRSA. One hundred and two of the MRSA strains were genetically evaluated. SCCmec genotypes were identified by M-PCR and the PVL gene (pvl) by end-point PCR. Resistance to erythromycin, rifampicin, clindamycin, and trimethoprim-sulfamethoxazole (SXT) was assessed by Kirby-Bauer disk diffusion method in accordance with the Clinical and Laboratory Standards Institute guidelines of 2012.

Results: Of the 102 strains evaluated, 97 (95%) were SCCmec type II, 5 (5%) were SCCmec type IVa, and all (100%) were pvl-negative. Resistance to erythromycin, clindamycin, rifampicin, and SXT was 97%, 95%, 0%, and 0%, respectively.

Conclusions: The prevalence of hospital-acquired MRSA was high. SCCmec type II was predominant and the pvl gene appeared not to play any role in the virulence of the MRSA strains from hospitalized children.

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墨西哥一家三级医院住院儿童的耐甲氧西林金黄色葡萄球菌SCCmec类型和pvl基因

背景:本研究旨在了解住院儿童耐甲氧西林金黄色葡萄球菌(MRSA)的流行情况、SCCmec类型、pton - valentine leukocidin (PVL)基因的存在以及对抗生素的敏感性。方法:2009年8月~ 2011年9月，从正常无菌体表分离金黄色葡萄球菌291株，其中MRSA 190株(65%)。对102株MRSA菌株进行了遗传评估。用M-PCR和终点PCR分别鉴定SCCmec基因型和PVL基因(PVL)。采用Kirby-Bauer盘片扩散法评估红霉素、利福平、克林霉素和甲氧苄啶-磺胺甲恶唑(SXT)的耐药性，并参照2012年临床与实验室标准学会指南。结果:102株检测菌株中，SCCmecⅱ型97株(95%)，SCCmec IVa型5株(5%)，pvl阴性(100%)。对红霉素、克林霉素、利福平和SXT的耐药率分别为97%、95%、0%和0%。结论:医院获得性MRSA感染率较高。SCCmec II型占主导地位，pvl基因在住院儿童MRSA菌株的毒力中似乎没有任何作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scandinavian Journal of Infectious Diseases 医学-传染病学

自引率

0.00%

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审稿时长

4-8 weeks