The influence of circulating blood volume on blood ethanol concentrations in a rat model.

Kumiko Asakura, Kyoko Maebashi, Masayoshi Ozawa, Sari Matsumoto, Tatsushige Fukunaga, Kimiharu Iwadate
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Abstract

In the medicolegal field, whether a victim was under the influence of ethanol at the time of an accident or injury is an important issue. However, trauma victims may have lost a large amount of blood, or received cardiopulmonary resuscitation or infusions of fluid or blood, making it difficult to interpret the ethanol concentration at the time of sampling. We, therefore, investigated changes in ethanol elimination in a rat model in which variations in circulating blood volume were induced by means of slow hemorrhage or infusion. Animals were divided into four experimental groups: hemorrhage (H), infusion (I), hemorrhage and infusion (H&I), and control (C). All rats were administered ethanol (1 g/kg body weight) intravenously over a period of 5 minutes. Blood (group C and group I: 0.2 ml, group H and group H&I: 0.5 ml/300gBW) was collected from rats in each group every 10 minutes for 4 h. Every 10 minutes after 30 minutes to 4 h of the ethanol administration, the rats in the infusion groups were administered saline (group I: 0.5 ml/300gBW, group H&I: 1.0 ml/300gBW). The concentration of ethanol in the blood samples was determined by using head-space gas chromatography. We found that the ethanol elimination rate did not differ between the groups, indicating that variations in body fluid due to bleeding or infusion have little to no effect on blood ethanol concentrations. Blood ethanol levels obtained after a prolonged state of shock or agonal, however, may need to be cautiously interpreted.

大鼠模型循环血容量对血乙醇浓度的影响。
在法医学领域,受害人在发生事故或受伤时是否受到乙醇的影响是一个重要问题。然而,创伤受害者可能失去了大量的血液,或接受了心肺复苏或输液或血液,这使得很难解释采样时的乙醇浓度。因此,我们在大鼠模型中研究了乙醇消除的变化,其中循环血容量的变化是通过缓慢出血或输液引起的。实验动物分为出血组(H)、输注组(I)、出血和输注组(H&I)和对照组(C)四个实验组。所有大鼠均静脉注射乙醇(1 g/kg体重),持续5分钟。各组大鼠每隔10分钟取血(C组和I组:0.2 ml, H组和H&I组:0.5 ml/300gBW),连续取血4 H。乙醇给药30 ~ 4 H后,每隔10分钟输注一次生理盐水(I组:0.5 ml/300gBW, H&I组:1.0 ml/300gBW)。采用顶空气相色谱法测定血液样品中乙醇浓度。我们发现两组之间的乙醇消除率没有差异,这表明由于出血或输液引起的体液变化对血液乙醇浓度几乎没有影响。然而,在长时间休克或昏迷状态后获得的血乙醇水平可能需要谨慎解释。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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