The Effect of Gynostemma pentaphyllum Extract on Mouse Dermal Fibroblasts.

ISRN Dermatology Pub Date : 2014-03-04 eCollection Date: 2014-01-01 DOI:10.1155/2014/202876

Sara Nadia Lobo, Yu Qing Qi, Quan Zhong Liu

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引用次数: 5

Abstract

Background. The objective of this paper is to demonstrate the effect of Gynostemma pentaphyllum extract on mouse dermal fibroblasts. Recent studies have shown that this plant may possess great antioxidant properties, which can be very beneficial in combating oxidative stress. Methods. Gynostemma pentaphyllum extract was prepared and mouse dermal fibroblasts were obtained and cultured as per our laboratory protocols. Twelve samples of cells were cultured under the same conditions and both negative and positive controls were established. Induction of oxidative stress was carried out using ultraviolet C (UVC) light. Viable cell count was carried out, using microscopy. The analysis of the overall results was processed using SPSS version 16.0. Results. Statistical analysis showed strong positive correlation between the concentration of Gynostemma pentaphyllum and the mean duration of cell viability (rs = 1), with a high level of statistical significance (P < 0.01). Likewise, strong positive correlation existed between trials of cell viability (rs = 0.988–1), with statistical significance (P < 0.01). Conclusion. Gynostemma pentaphyllum extract prolongs viability of mouse dermal fibroblasts damaged by UVC light-induced oxidative stress. The results show the potential benefits of this extract on dermal cell aging.

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绞股蓝提取物对小鼠真皮成纤维细胞的影响。

背景。本文旨在探讨绞股蓝提取物对小鼠真皮成纤维细胞的影响。最近的研究表明，这种植物可能具有很强的抗氧化特性，这对对抗氧化应激非常有益。方法。制备绞股蓝提取物，获得小鼠真皮成纤维细胞，并按照实验方案进行培养。在相同条件下培养12个细胞样本，建立阴性对照和阳性对照。采用紫外C (UVC)光诱导氧化应激。在显微镜下进行活细胞计数。总体结果的分析采用SPSS 16.0版本进行。结果。经统计学分析，绞股蓝浓度与细胞平均存活时间呈极显著正相关(rs = 1)，具有高度统计学意义(P < 0.01)。细胞活力试验间也存在较强的正相关(rs = 0.988-1)，差异均有统计学意义(P < 0.01)。结论。绞股蓝提取物可延长UVC光诱导氧化应激损伤小鼠真皮成纤维细胞的活力。结果表明，该提取物对真皮细胞衰老具有潜在的益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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ISRN Dermatology

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