Frequency and Spectrum of KRAS Mutations in Moroccan Patients with Lung Adenocarcinoma.

ISRN oncology Pub Date : 2014-03-05 eCollection Date: 2014-01-01 DOI:10.1155/2014/192493
Ibrahim Elghissassi, Hanane Inrhaoun, Anwar Boukir, Fouad Kettani, Lamia Gamra, Amina Mestari, Lamia Jabri, Youssef Bensouda, Hind Mrabti, Hassan Errihani
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引用次数: 5

Abstract

Background. In lung adenocarcinoma, the frequency of KRAS mutations is ethnicity dependent with a higher proportion in African Americans and white Caucasians than in Asians. The prevalence of these mutations among North Africans patients is unknown. The objective of this study was to report the frequency and spectrum of KRAS mutations in a group of Moroccan lung adenocarcinoma patients. Methods. Tumor specimens from 117 Moroccan patients with lung adenocarcinoma were selected to determine frequency and spectrum of KRAS mutations. KRAS mutations in codons 12 and 13 of exon 2 were analyzed using conventional DNA sequencing. Results. The overall frequency of the KRAS mutations was 9% (11/117). In the population with KRAS mutations, there was a trend towards more male (P = 0.06) and more smokers (P = 0.08) compared to patients with wild type KRAS. KRAS mutations were located at codon 12 in 10 out of 11 patients (91%). The G12C mutation was the most frequent KRAS mutation (73%). Conclusion. This is the first study to date examining the frequency and spectrum of KRAS mutations in lung adenocarcinomas in North African and Arab populations. KRAS mutation frequency in Moroccan patients was comparable with the frequency observed in East-Asian population. KRAS mutations are more likely observed in males and smokers and to be transversions. Further studies, in larger numbers of patients, are needed to confirm these findings.

摩洛哥肺腺癌患者KRAS突变的频率和谱。
背景。在肺腺癌中,KRAS突变的频率与种族有关,非裔美国人和白种人的比例高于亚洲人。这些突变在北非患者中的流行程度尚不清楚。本研究的目的是报告一组摩洛哥肺腺癌患者中KRAS突变的频率和谱。方法。选取117例摩洛哥肺腺癌患者的肿瘤标本,测定KRAS突变的频率和谱。采用常规DNA测序方法分析2外显子12和13密码子的KRAS突变。结果。KRAS突变的总频率为9%(11/117)。KRAS突变人群中男性比野生型KRAS多(P = 0.06),吸烟者多(P = 0.08)。11例患者中有10例(91%)KRAS突变位于密码子12。G12C突变是最常见的KRAS突变(73%)。结论。这是迄今为止第一个检测北非和阿拉伯人群肺腺癌中KRAS突变频率和谱的研究。摩洛哥患者的KRAS突变频率与东亚人群中观察到的频率相当。KRAS突变更可能在男性和吸烟者中观察到,并且是易位的。需要在更多患者中进行进一步的研究来证实这些发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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