[Establishment of localization ultrathin section for cytopathic cells].

Abstract

Objective: To establish a localization ultrathin section method through which target cytopathic cells could be sectioned in situ.

Methods: Lab-Tek Chamber slide system (177402) was selected as resin embedding mould. Cells infected with Human adenovirus type 5 (Ad5) or A/HN/SWL3/ 2009 (H1N1) influenza virus were embedded in situ as models. Target cytopathic cells were exposed by trimming, sectioned and observed under transmission electron microscope (TEM).

Results: Target cells could be sectioned in situ and virus particles could be found easily on sections.

Conclusion: A localization ultrathin sectioning method was established and this technique could be applied in virus detection in cytopathic cells to improve TEM detection efficiency.