[Relationship between serum HBV DNA level and follicular helper T lymphocyte in patients with chronic hepatitis B and its significance].

中华实验和临床病毒学杂志 Pub Date : 2013-10-01

Juan-Hua Wang, Xi-Bing Gu, Yin-Fang Zhu, Zhong Hua, Dong Wang, Xiao-Juan Yang, Yue-Qin Xu, Zhong-Hua Lu

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引用次数: 0

Abstract

Objective: To explore relationship between HBV DNA level and peripheral blood follicular helper T lymphocyte (Tfh) in patients with chronic hepatitis B (CHB) and its significance.

Methods: HBV DNA levels of 179 cases of CHB patients with positive HBV DNA, positive HBeAg and positive human leukocyte antigen(HLA)-A2 were tested with real time fluorescent quantitative PCR. Tfh and HBV specific CTL were tested with flow cytometry. IL-21 was also tested. 179 cases of CHB patients were divided into group A and group B based on HBV DNA levels, 86 cases in group A, HBV DNA levels were 10(4)-10(5) copies/ml, 93 cases in group B, HBV DNA levels were 10(6)-10(7) copies/ml. Above testing indexes of the two groups were compared.

Results: HBV DNA levels of group A were (4.85 +/- 0.37) log10 copies/ml, HBV DNA levels of group B were (6.83 +/- 0.31 ) log10 copies/ml, t = 27.31, P < 0. 001; Tfh of group A was (5.96 +/- 1.59)%, higher than that of group B (3.71 +/- 2.15)%, t = 4.92, P < 0.01; IL-21 of group A was (42.61 +/- 15.11)ng/L, higher than that of group B (14.91 +/- 3.15) ng/L, t = 8.62, P < 0.01; HBV specific CTL of group A was (0.36 +/- 0.08)%, higher than that of group B (0.18 +/- 0.06)%, t = 19.99, P < 0.001.

Conclusion: Serum HBV DNA level of CHB patients is related to the level of peripheral blood Tfh level: patients with low HBV DNA level have high Tfh level, high IL-21 level and high HBV specific CTL level. Patients with high HBV DNA level have low Tfh level, low IL-21 level and low HBV specific CTL level. The mechanism of baseline HBV DNA level affecting anti-viral therapy may be related to Tfh level.

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[慢性乙型肝炎患者血清HBV DNA水平与滤泡性T淋巴细胞的关系及意义]。

目的:探讨慢性乙型肝炎(CHB)患者外周血滤泡辅助性T淋巴细胞(Tfh)与HBV DNA水平的关系及其意义。方法:采用实时荧光定量PCR检测179例HBV DNA阳性、HBeAg阳性、人白细胞抗原(HLA)-A2阳性的CHB患者HBV DNA水平。流式细胞术检测Tfh和HBV特异性CTL。IL-21也进行了检测。根据HBV DNA水平将179例CHB患者分为A组和B组，A组86例，HBV DNA水平为10(4)~ 10(5)copies/ml, B组93例，HBV DNA水平为10(6)~ 10(7)copies/ml。比较两组患者的上述检测指标。结果:A组HBV DNA水平为(4.85 +/- 0.37)log10 copies/ml, B组HBV DNA水平为(6.83 +/- 0.31)log10 copies/ml, t = 27.31, P < 0。001;A组Tfh为(5.96 +/- 1.59)%，高于B组(3.71 +/- 2.15)%，t = 4.92, P < 0.01;A组IL-21为(42.61 +/- 15.11)ng/L，高于B组(14.91 +/- 3.15)ng/L, t = 8.62, P < 0.01;A组HBV特异性CTL为(0.36 +/- 0.08)%，高于B组(0.18 +/- 0.06)%，t = 19.99, P < 0.001。结论:慢性乙型肝炎患者血清HBV DNA水平与外周血Tfh水平相关:HBV DNA水平低的患者Tfh水平高，IL-21水平高，HBV特异性CTL水平高。HBV DNA水平高的患者Tfh水平低，IL-21水平低，HBV特异性CTL水平低。HBV DNA基线水平影响抗病毒治疗的机制可能与Tfh水平有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中华实验和临床病毒学杂志

CiteScore

0.20

自引率

0.00%

发文量

4549