Prolactin is a potential physiological modulator of swine ovarian follicle function

Regulatory Peptides Pub Date : 2014-02-10 DOI:10.1016/j.regpep.2014.01.003

Giuseppina Basini, Laura Baioni, Simona Bussolati, Stefano Grolli, Francesca Grasselli

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引用次数: 17

Abstract

Apart from the well established role of prolactin (PRL) in the control of mammary development and lactation, this hormone appears to possess a variety of physiological functions and evidence exists about its expression in many extra-pituitary sites.

This experimental work was undertaken to gain knowledge about PRL and its receptor presence in the porcine antral follicle. In particular, we investigated the expression and local production of PRL in follicular fluid, theca and granulosa cells cultured in standard conditions and in hypoxia. Then, we also investigated its modulatory effect on several parameters mainly involved in granulosa cell function, namely redox status and steroidogenesis. In order to verify an involvement of PRL in the control of ovarian angiogenesis, a process strictly linked to follicle growth and development, we have verified possible PRL effects on granulosa cell production of Vascular Endothelial Growth Factor (VEGF) and nitric oxide as well as its modulatory role on the angiogenic activity of endothelial cells.

Our data demonstrate that in the swine PRL is expressed in both components of the antral follicle, theca and granulosa layers, and it is produced by granulosa cells. Moreover, the hormone represents a relevant modulatory factor on key processes underlying follicular growth and development, such as steroidogenesis and angiogenesis.

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催乳素是猪卵巢卵泡功能的潜在生理调节剂

泌乳素(PRL)除了在控制乳腺发育和泌乳中的作用外，这种激素似乎具有多种生理功能，并且有证据表明它在许多垂体外部位表达。本实验工作是为了了解PRL及其受体在猪窦卵泡中的存在。我们特别研究了在标准条件和缺氧条件下培养的卵泡液、卵泡膜和颗粒细胞中PRL的表达和局部产生。然后，我们还研究了其对颗粒细胞功能主要涉及的几个参数的调节作用，即氧化还原状态和类固醇生成。为了验证PRL参与卵巢血管生成的控制，这一过程与卵泡的生长发育密切相关，我们验证了PRL可能对颗粒细胞生成血管内皮生长因子(VEGF)和一氧化氮的影响，以及其对内皮细胞血管生成活性的调节作用。我们的数据表明，在猪中，PRL在窦卵泡、卵膜和颗粒层的两种成分中都有表达，并且由颗粒细胞产生。此外，这种激素在卵泡生长和发育的关键过程中是一种相关的调节因子，如类固醇生成和血管生成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.