Michael F. Jarvis
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引用次数: 4
Abstract
The purine nucleoside adenosine (ADO) is an important modulator of cellular function in mammalian tissues, modulating cellular function and neuronal excitability via interactions with different cell surface receptor subtypes that are heterogeneously distributed in both the mammalian CNS and peripheral tissues. Four ADO receptor subtypes have been cloned and characterized. Described in this unit are three radioligand binding assays for pharmacological characterization of the high-affinity ADO receptor subtypes A1, A2A, and A3 receptors. Pharmacological characterization of the low-affinity A2B receptor has been enabled by the use of tritiated xanthine PSB-603. Because receptor localization is an important criterion for differentiation of receptor subtypes, a support protocol that describes the methodology for the localization of ADO receptors in rat brain tissue using autoradiography is also included. Curr. Protoc. Pharmacol. 62:1.9.1-1.9.16. © 2013 by John Wiley & Sons, Inc.
P1(腺苷)嘌呤受体的表征
嘌呤核苷腺苷(嘌呤核苷腺苷，ADO)是哺乳动物组织中重要的细胞功能调节剂，通过与分布在哺乳动物中枢神经系统和外周组织中的不同细胞表面受体亚型相互作用，调节细胞功能和神经元兴奋性。已经克隆并鉴定了四种ADO受体亚型。本单元描述了用于高亲和ADO受体A1、A2A和A3受体亚型药理学表征的三种放射性配体结合试验。通过使用氚化黄嘌呤PSB-603，低亲和力A2B受体的药理学特征得以实现。由于受体定位是受体亚型分化的重要标准，本文还包括了一项支持协议，该协议描述了使用放射自显影技术在大鼠脑组织中定位ADO受体的方法。咕咕叫。Protoc。杂志,62:1.9.1-1.9.16。©2013 by John Wiley &儿子,Inc。
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