Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)

Current Protocols in Microbiology Pub Date : 2018-02-16 DOI:10.1002/9780471729259.mc09d03s30

Yoann Le Breton, Kevin S. McIver

引用次数: 40

Abstract

Streptococcus pyogenes (the Group A Streptococcus, GAS) is a Gram-positive bacterium responsible for a wide spectrum of diseases ranging from mild superficial infections (pharyngitis, impetigo) to severe, often life-threatening invasive diseases (necrotizing fasciitis, streptococcal toxic shock syndrome) in humans. This unit describes molecular techniques for the genetic manipulation of S. pyogenes with detailed protocols for transformation, gene disruption, allelic exchange, transposon mutagenesis, and genetic complementation. Curr. Protoc. Microbiol. 30:9D.3.1-9D.3.29. © 2013 by John Wiley & Sons, Inc.

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化脓性链球菌(A群链球菌，GAS)的遗传操作

化脓性链球菌(A群链球菌，GAS)是一种革兰氏阳性细菌，可导致多种疾病，从轻度浅表感染(咽炎、脓疱疮)到严重的、通常危及生命的侵袭性疾病(坏死性筋膜炎、链球菌中毒性休克综合征)。本单元描述了化脓性葡萄球菌遗传操作的分子技术，并详细介绍了转化、基因破坏、等位基因交换、转座子诱变和遗传互补的方案。咕咕叫。Protoc。Microbiol 30:9D.3.1-9D.3.29。©2013 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.