BioID: A Screen for Protein-Protein Interactions

Q1 Biochemistry, Genetics and Molecular Biology
Kyle J. Roux, Dae In Kim, Brian Burke
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引用次数: 201

Abstract

BioID is a unique method to screen for physiologically relevant protein interactions that occur in living cells. This technique harnesses a promiscuous biotin ligase to biotinylate proteins based on proximity. The ligase is fused to a protein of interest and expressed in cells, where it biotinylates proximal endogenous proteins. Because it is a rare protein modification in nature, biotinylation of these endogenous proteins by BioID fusion proteins enables their selective isolation and identification with standard biotin-affinity capture. Proteins identified by BioID are candidate interactors for the protein of interest. BioID can be applied to insoluble proteins, can identify weak and/or transient interactions, and is amenable to temporal regulation. Initially applied to mammalian cells, BioID has potential application in a variety of cell types from diverse species. Curr. Protoc. Protein Sci. 74:19.23.1-19.23.14. © 2013 by John Wiley & Sons, Inc.

BioID:蛋白质-蛋白质相互作用的筛选
BioID是一种筛选活细胞中发生的生理相关蛋白质相互作用的独特方法。该技术利用混杂生物素连接酶与生物素酸蛋白的接近性。连接酶与感兴趣的蛋白质融合并在细胞中表达,在细胞中对近端内源性蛋白质进行生物素化。由于这在自然界是一种罕见的蛋白质修饰,通过BioID融合蛋白对这些内源性蛋白质进行生物素化,使它们能够通过标准生物素亲和力捕获进行选择性分离和鉴定。BioID鉴定的蛋白是目标蛋白的候选相互作用物。BioID可以应用于不溶性蛋白质,可以识别弱和/或瞬态相互作用,并且可以接受时间调节。BioID最初应用于哺乳动物细胞,在来自不同物种的多种细胞类型中具有潜在的应用前景。咕咕叫。Protoc。蛋白质科学,74:19.23.1-19.23.14。©2013 by John Wiley &儿子,Inc。
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来源期刊
Current Protocols in Protein Science
Current Protocols in Protein Science Biochemistry, Genetics and Molecular Biology-Biochemistry
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期刊介绍: With the mapping of the human genome, more and more researchers are exploring protein structures and functions in living organisms. Current Protocols in Protein Science provides protein scientists, biochemists, molecular biologists, geneticists, and others with the first comprehensive suite of protocols for this growing field.
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