A Mouse Model of Foodborne Listeria monocytogenes Infection

Current Protocols in Microbiology Pub Date : 2018-02-16 DOI:10.1002/9780471729259.mc09b03s31

Elsa N. Bou Ghanem, Tanya Myers-Morales, Sarah E.F. D'Orazio

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引用次数: 25

Abstract

Listeria monocytogenes causes foodborne disease in humans that ranges in severity from mild, self-limiting gastroenteritis to life-threatening systemic infections of the blood, brain, or placenta. The most commonly used animal model of listeriosis is intravenous infection of mice. This systemic model is highly reproducible, and thus, useful for studying cell-mediated immune responses against an intracellular bacterial pathogen, but it completely bypasses the gastrointestinal phase of L. monocytogenes infection. Intragastric inoculation of L. monocytogenes produces more variable results and may cause direct bloodstream invasion in some animals. The foodborne transmission model described here does not require specialized skills to perform and results in infections that more closely mimic human disease. This natural feeding model can be used to study both the host- and pathogen-derived factors that govern susceptibility or resistance to orally acquired L. monocytogenes. Curr. Protoc. Microbiol. 31:9B.3.1-9B.3.16. © 2013 by John Wiley & Sons, Inc.

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食源性单核细胞增生李斯特菌感染小鼠模型的建立

单核细胞增生李斯特菌在人类中引起食源性疾病，其严重程度从轻度自限性胃肠炎到危及生命的全身血液、脑或胎盘感染不等。李斯特菌病最常用的动物模型是小鼠静脉感染。该系统模型具有高度可重复性，因此可用于研究细胞介导的针对细胞内细菌病原体的免疫反应，但它完全绕过了单核增生乳杆菌感染的胃肠道期。在一些动物胃内接种单核细胞增生乳杆菌会产生更多不同的结果，并可能导致直接血流侵入。这里描述的食源性传播模型不需要专门的技能来执行，并且导致更接近人类疾病的感染。这种自然摄食模型可用于研究控制口服获得性单核细胞增生乳杆菌易感性或耐药性的宿主和病原体来源因素。咕咕叫。Protoc。Microbiol 31:9B.3.1-9B.3.16。©2013 by John Wiley &儿子,Inc。

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Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.