Listeria monocytogenes: Cultivation and Laboratory Maintenance

Grant S. Jones, Sarah E.F. D'Orazio
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引用次数: 34

Abstract

This unit describes general procedures for the lab cultivation and storage of the Gram-positive facultative intracellular bacterium Listeria monocytogenes. The basic protocols are relevant for a wide scope of applications including microbial genetics and both in vitro and in vivo infection studies. Commonly used L. monocytogenes strains, serotypes, and growth parameters are also discussed. Curr. Protoc. Microbiol. 31:9B.2.1-9B.2.7. ©2013 by John Wiley & Sons, Inc.

Abstract Image

单核增生李斯特菌:培养和实验室维护
本单元描述了实验室培养和储存革兰氏阳性兼性单核细胞增生李斯特菌的一般程序。基本方案适用于广泛的应用,包括微生物遗传学和体外和体内感染研究。还讨论了常用的单核增生乳杆菌菌株、血清型和生长参数。咕咕叫。Protoc。Microbiol 31:9B.2.1-9B.2.7。©2013 by John Wiley &儿子,Inc。
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来源期刊
Current Protocols in Microbiology
Current Protocols in Microbiology Immunology and Microbiology-Parasitology
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期刊介绍: Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.
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