Kari Debbink, Veronica Costantini, Jesica Swanstrom, Sudhakar Agnihothram, Jan Vinjé, Ralph Baric, Lisa Lindesmith
下载PDF
{"title":"Human Norovirus Detection and Production, Quantification, and Storage of Virus-Like Particles","authors":"Kari Debbink, Veronica Costantini, Jesica Swanstrom, Sudhakar Agnihothram, Jan Vinjé, Ralph Baric, Lisa Lindesmith","doi":"10.1002/9780471729259.mc15k01s31","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Human noroviruses constitute a significant worldwide disease burden. Each year, noroviruses cause over 267 million infections, deaths in over 200,000 children under the age of five, and over 50% of U.S. food-borne illness. Due to the absence of a tissue culture model or small animal model to study human norovirus, virus-like particles (VLPs) and ELISA-based biological assays have been used to answer questions about norovirus evolution and immunity as well to provide a potential vaccine platform. This chapter outlines the protocols for norovirus detection in stool, as well as norovirus VLP design, production, purification, and storage using a Venezuelan equine encephalitis virus (VEE)–based virus replicon particle (VRP) expression system. <i>Curr. Protoc. Microbiol</i>. 31:15K.1.1-15K.1.45. © 2013 by John Wiley & Sons, Inc.</p></div>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780471729259.mc15k01s31","citationCount":"30","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/9780471729259.mc15k01s31","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 30
引用
批量引用
Abstract
Human noroviruses constitute a significant worldwide disease burden. Each year, noroviruses cause over 267 million infections, deaths in over 200,000 children under the age of five, and over 50% of U.S. food-borne illness. Due to the absence of a tissue culture model or small animal model to study human norovirus, virus-like particles (VLPs) and ELISA-based biological assays have been used to answer questions about norovirus evolution and immunity as well to provide a potential vaccine platform. This chapter outlines the protocols for norovirus detection in stool, as well as norovirus VLP design, production, purification, and storage using a Venezuelan equine encephalitis virus (VEE)–based virus replicon particle (VRP) expression system. Curr. Protoc. Microbiol . 31:15K.1.1-15K.1.45. © 2013 by John Wiley & Sons, Inc.
人诺如病毒的检测及病毒样颗粒的生产、定量和储存
人类诺如病毒在世界范围内构成重大疾病负担。每年，诺如病毒导致超过2.67亿人感染，20多万5岁以下儿童死亡，以及超过50%的美国食源性疾病。由于缺乏组织培养模型或小动物模型来研究人类诺如病毒，病毒样颗粒(vlp)和基于elisa的生物测定已被用于回答有关诺如病毒进化和免疫的问题，并提供潜在的疫苗平台。本章概述了粪便中诺如病毒检测的方案，以及使用委内瑞拉马脑炎病毒(VEE)为基础的病毒复制子颗粒(VRP)表达系统设计、生产、纯化和储存诺如病毒VLP。咕咕叫。Protoc。Microbiol 31:15K.1.1-15K.1.45。©2013 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
