Kari Debbink, Veronica Costantini, Jesica Swanstrom, Sudhakar Agnihothram, Jan Vinjé, Ralph Baric, Lisa Lindesmith
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Abstract
Human noroviruses constitute a significant worldwide disease burden. Each year, noroviruses cause over 267 million infections, deaths in over 200,000 children under the age of five, and over 50% of U.S. food-borne illness. Due to the absence of a tissue culture model or small animal model to study human norovirus, virus-like particles (VLPs) and ELISA-based biological assays have been used to answer questions about norovirus evolution and immunity as well to provide a potential vaccine platform. This chapter outlines the protocols for norovirus detection in stool, as well as norovirus VLP design, production, purification, and storage using a Venezuelan equine encephalitis virus (VEE)–based virus replicon particle (VRP) expression system. Curr. Protoc. Microbiol . 31:15K.1.1-15K.1.45. © 2013 by John Wiley & Sons, Inc.
人诺如病毒的检测及病毒样颗粒的生产、定量和储存
人类诺如病毒在世界范围内构成重大疾病负担。每年,诺如病毒导致超过2.67亿人感染,20多万5岁以下儿童死亡,以及超过50%的美国食源性疾病。由于缺乏组织培养模型或小动物模型来研究人类诺如病毒,病毒样颗粒(vlp)和基于elisa的生物测定已被用于回答有关诺如病毒进化和免疫的问题,并提供潜在的疫苗平台。本章概述了粪便中诺如病毒检测的方案,以及使用委内瑞拉马脑炎病毒(VEE)为基础的病毒复制子颗粒(VRP)表达系统设计、生产、纯化和储存诺如病毒VLP。咕咕叫。Protoc。Microbiol 31:15K.1.1-15K.1.45。©2013 by John Wiley &儿子,Inc。
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