{"title":"White spot syndrome virus Manipulates Ubiquitin Gene Expression in Penaeus monodon.","authors":"R Vidya, P Gireesh-Babu, K Pani Prasad","doi":"10.1007/s13337-012-0113-0","DOIUrl":null,"url":null,"abstract":"<p><p>The present study reports the effect of WSSV on the expression profiles of shrimp ubiquitin gene both at protein and transcript levels at different time points after infection. A gradual decrease in ubiquitin protein levels was observed till 12 hpi (0.25 and 0.23 μg/mg of the total protein in gills and muscle, respectively) with a sudden increase at 18 hpi (1.86 and 1.8 μg/mg of total protein in gills and muscle, respectively). The RT-PCR results showed a gradual decrease in ubiquitin mRNA levels in gill tissue till 12 hpi while a more or less constant mRNA levels were observed in muscle tissue till 12 hpi. However, a fourfold and 16 fold induction in ubiquitin mRNA levels at 18 hpi in gill and muscle, respectively suggests its possible regulation by WSSV in the manifestation of the disease. </p>","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"82-4"},"PeriodicalIF":0.0000,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0113-0","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Virology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s13337-012-0113-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2012/12/16 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 8
Abstract
The present study reports the effect of WSSV on the expression profiles of shrimp ubiquitin gene both at protein and transcript levels at different time points after infection. A gradual decrease in ubiquitin protein levels was observed till 12 hpi (0.25 and 0.23 μg/mg of the total protein in gills and muscle, respectively) with a sudden increase at 18 hpi (1.86 and 1.8 μg/mg of total protein in gills and muscle, respectively). The RT-PCR results showed a gradual decrease in ubiquitin mRNA levels in gill tissue till 12 hpi while a more or less constant mRNA levels were observed in muscle tissue till 12 hpi. However, a fourfold and 16 fold induction in ubiquitin mRNA levels at 18 hpi in gill and muscle, respectively suggests its possible regulation by WSSV in the manifestation of the disease.
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