A Highly Thermostable Xylanase from Stenotrophomonas maltophilia: Purification and Partial Characterization.

Q2 Biochemistry, Genetics and Molecular Biology
Enzyme Research Pub Date : 2013-01-01 Epub Date: 2013-12-14 DOI:10.1155/2013/429305
Abhay Raj, Sharad Kumar, Sudheer Kumar Singh
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引用次数: 36

Abstract

Seven xylanolytic bacterial strains were isolated from saw-dust dump soil. The bacterial strain X6 was selected on the basis of the highest xylanase activity with no cellulase contamination. It was identified as Stenotrophomonas maltophilia by biochemical tests and 16S rRNA gene sequencing approach. Xylanase production studies by S. maltophilia on different commercial xylans and agro-industrial residues suggested that wheat bran was the best carbon source for xylanase production (26.4 ± 0.6 IU/mL). The studies with inorganic and organic nitrogen sources suggested yeast extract as the best support for xylanase production (25 ± 0.6 IU/mL). Maximum xylanase production was observed at initial medium pH = 8.0 (23.8 ± 0.4 IU/mL) with production at pH = 7.0 and pH = 9.0 being almost comparable. Xylanase produced by S. maltophilia was purified to homogeneity using ammonium sulfate precipitation, gel filtration, and ion exchange chromatography. The final purification was 5.43-fold with recovery of 19.18%. The molecular weight of the purified xylanase protein was ~142 kDa. Both crude and purified xylanase had good stability at pH = 9.0 and 80°C with activity retention greater than 90% after 30 min incubation. The enzyme stability at high temperature and alkaline pH make it potentially effective for industrial applications.

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嗜麦芽寡养单胞菌的高耐热木聚糖酶:纯化和部分特性。
从木屑排土场土壤中分离到7株解木质素细菌。菌株X6的木聚糖酶活性最高,且无纤维素酶污染。经生化检测和16S rRNA基因测序鉴定为嗜麦芽窄养单胞菌。嗜麦芽酵母对不同商品木聚糖和农工废渣产木聚糖酶的研究表明,麦麸是产木聚糖酶的最佳碳源(26.4±0.6 IU/mL)。无机氮源和有机氮源的研究表明,酵母提取物(25±0.6 IU/mL)是产木聚糖酶的最佳载体。初始培养基pH = 8.0(23.8±0.4 IU/mL)时木聚糖酶产量最高,pH = 7.0和pH = 9.0时产量几乎相当。通过硫酸铵沉淀、凝胶过滤和离子交换层析纯化了嗜麦芽葡萄球菌产生的木聚糖酶。最终纯化率为5.43倍,回收率为19.18%。纯化得到的木聚糖酶蛋白分子量为~142 kDa。粗木聚糖酶和纯化木聚糖酶在pH = 9.0和80℃条件下均具有良好的稳定性,孵育30 min后活性保持率均大于90%。酶在高温和碱性条件下的稳定性使其具有潜在的工业应用价值。
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来源期刊
Enzyme Research
Enzyme Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
4.60
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0.00%
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