The inhibitory effect of anandamide on oxytocin and vasopressin secretion from neurohypophysis is mediated by nitric oxide

Regulatory Peptides Pub Date : 2014-01-10 DOI:10.1016/j.regpep.2013.12.004

Valeria Luce , Javier Fernandez Solari , Valeria Rettori , Andrea De Laurentiis

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引用次数: 18

Abstract

The neurohypophyseal hormones oxytocin (OT) and vasopressin (VP) are involved in behavioral, autonomic and neuroendocrine functions. Both peptides are synthesized in magnocellular neurons of paraventricular and supraoptic nuclei at hypothalamic level whose axons terminate in the neurohypophysis (NH), from where OT and VP are released into the systemic circulation. NH contains abundant nitric oxide (NO) synthase suggesting that NO plays a role in the release of these neuropeptides. The endocannabinoid system is present in magnocellular neurons of the hypothalamic neurohypophyseal system, and we have previously demonstrated that endocannabinoids modulate OT secretion at hypothalamic level.

In the present work, we investigated the in vitro effect of the endocannabinoid anandamide (AEA) on OT and VP release from NH of untreated adult male rats and the involvement of NO in this action.

Our results showed that AEA decreased OT and VP secretion from NH. AEA action was mediated by NO, since the inhibition of NO synthesis completely blocked this inhibitory effect. We found that cannabinoid receptor type 2 (CB₂) and transient receptor potential cation channel subfamily V member 1 (TRPV1) are involved in the inhibitory effect of AEA because AM630 and capsazepine, CB₂ and TRPV1 antagonists respectively, but not AM251, a CB₁ antagonist, blocked AEA effect at neurohypophyseal level.

These findings revealed an interaction between endocannabinoid, nitric oxide and oxytocin/vasopressin systems that could be involved in the modulation of homeostatic, behavioral and reproductive processes.

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anandamide对神经垂体后叶催产素和加压素分泌的抑制作用是由一氧化氮介导的

神经垂体激素催产素(OT)和加压素(VP)参与行为、自主神经和神经内分泌功能。这两种多肽都是在下丘脑水平的室旁核和视上核的大细胞神经元中合成的，其轴突终止于神经垂体(NH)，从这里OT和VP被释放到体循环中。NH含有丰富的一氧化氮(NO)合成酶，提示NO在这些神经肽的释放中起作用。内源性大麻素系统存在于下丘脑神经垂体系统的大细胞神经元中，我们之前已经证明内源性大麻素在下丘脑水平调节OT的分泌。在本研究中，我们研究了内源性大麻素anandamide (AEA)对未处理的成年雄性大鼠体外释放OT和VP的影响以及NO参与这一作用。我们的研究结果表明，AEA降低了NH中OT和VP的分泌。AEA的作用是由NO介导的，因为抑制NO的合成完全阻断了这种抑制作用。我们发现，大麻素受体2型(CB2)和瞬时受体电位阳离子通道亚家族V成员1 (TRPV1)参与了AEA的抑制作用，因为AM630和辣椒素分别是CB2和TRPV1拮抗剂，而CB1拮抗剂AM251在神经垂体水平上阻断了AEA的作用。这些发现揭示了内源性大麻素、一氧化氮和催产素/抗利尿激素系统之间的相互作用，可能参与调节体内平衡、行为和生殖过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

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审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.