Diversity of Hepatitis C virus in Southern India Based on 5'UTR Sequence.

Indian Journal of Virology Pub Date : 2012-12-01 Epub Date: 2012-09-25 DOI:10.1007/s13337-012-0103-2

R Amjesh, Achuthsankar S Nair, V S Sugunan

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引用次数: 3

Abstract

Hepatitis C virus (HCV) exhibits genotype-specific variations in geographical distribution as a consequence of drug and immune induced evolution. Present study was aimed at discerning the distribution and prevalence of the various genotypes and subtypes of HCV in southern India. The HCV positive patient's serum was collected from different hospitals and blood banks from the states of Kerala, Tamil Nadu, Andhra Pradesh and Karnataka. Among 114 HCV positive samples, we could find only 44 isolates that are found both positive in ELISA and RT-PCR. From these samples 5' untranslated region (5'UTR) were amplified, sequenced and sub typed. Analysis of 5'UTR region of the 44 isolates shows that, genotypes 1, 3, 4 and 6 are present with genotype 3 being the most frequent. The present study shows that HCV genotype 3 subtype B was the most prevalent, forming 47.7 % among the population in southern India. The present study urges for discovering novel therapeutic agents that should be specific to genotype 3 subtype B, for the management of HCV in southern India.

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基于5'UTR序列的印度南部丙型肝炎病毒多样性

由于药物和免疫诱导的进化，丙型肝炎病毒(HCV)在地理分布上表现出基因型特异性变异。本研究旨在了解印度南部HCV各种基因型和亚型的分布和流行情况。该HCV阳性患者的血清是从喀拉拉邦、泰米尔纳德邦、安得拉邦和卡纳塔克邦的不同医院和血库收集的。在114份HCV阳性样本中，ELISA和RT-PCR均为阳性的只有44株。从这些样本中扩增5'非翻译区(5' utr)，测序并分型。44株菌株的5′utr区分析显示，存在基因1、3、4和6型，其中基因3型最为常见。目前的研究表明，HCV基因3亚型B最普遍，在印度南部人群中占47.7%。目前的研究敦促发现针对基因3型B亚型的新型治疗药物，用于管理印度南部的HCV。

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来源期刊

Indian Journal of Virology 医学-病毒学

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0.00%

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审稿时长

6-12 weeks