Corticotropin-releasing factor-like peptide modifies the AMPA-, NMDA-dependent and GABAB-ergic properties of synaptic transmissions in vitro

Regulatory Peptides Pub Date : 2014-01-10 DOI:10.1016/j.regpep.2013.11.001

Anatoly A. Mokrushin

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引用次数: 2

Abstract

The aim of this study was to investigate the neurotrophic effects of the mystixin-7 mini-peptide (MTX, a synthetic corticotrophin-releasing-factor-like peptide-like peptide) using a slice-based system. The technique on-line monitoring of electrophysiological parameters (excitatory glutamatergic AMPAR-, NMDAR-dependent and inhibitory GABA_B-ergic postsynaptic mechanisms) in the olfactory cortex slices of the rat brain exposed to varied amounts of MTX was used. MTX in a dose-dependent manner inhibited both the AMPAR- and NMDAR-mediated postsynaptic processes. The peptide caused depression of inhibitory GABA_B-ergic processes only at low doses of MTX (10, 25, 50 mg/mL) while at higher doses (100, 250 mg/mL) it enhanced them. These effects of MTX were reversible. AMPA-dependent (but not NMDA-mediated mechanisms) and inhibitory processes were restored after washing.

Triple reperfusion of slices with MTX (100 mg/mL) accelerated the inhibitory processes and induced NMDAR desensitization. MTX evoked the long-term depression on θ burst stimulation of the slices. This study did not only lead to the conclusion that the functions of the MTX mini-peptide is not limited to anti-inflammatory effects, but also is included modifications of excitatory glutamatergic AMPAR-, NMDAR-dependent and inhibitory GABA_B-ergic postsynaptic mechanisms.

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促肾上腺皮质激素释放因子样肽改变体外突触传递的AMPA-， nmda依赖性和gabab能特性

本研究的目的是利用基于切片的系统研究神秘素-7微肽(MTX，一种合成的促肾上腺皮质激素释放因子样肽样肽)的神经营养作用。采用在线监测不同剂量MTX暴露大鼠脑嗅皮质片电生理参数(兴奋性谷氨酸能AMPAR-、nmdar依赖和抑制性gabab -能突触后机制)的技术。MTX以剂量依赖的方式抑制AMPAR和nmdar介导的突触后过程。该肽仅在低剂量MTX(10、25、50 mg/mL)下引起抑制性gabab -能过程的抑制，而在高剂量(100、250 mg/mL)下则增强了抑制性gabab -能过程。甲氨蝶呤的这些作用是可逆的。洗涤后，ampa依赖(但不是nmda介导的机制)和抑制过程恢复。MTX (100 mg/mL)三次再灌注切片加速了抑制过程，诱导NMDAR脱敏。MTX对脑片θ爆发刺激产生长期抑制作用。本研究不仅得出结论，MTX微肽的功能不仅限于抗炎作用，还包括改变兴奋性谷氨酸- AMPAR-、nmda -依赖和抑制性gabab -能突触后机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

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审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.

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