A multitasking Argonaute: exploring the many facets of C. elegans CSR-1.

Christopher J Wedeles, Monica Z Wu, Julie M Claycomb
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引用次数: 27

Abstract

While initial studies of small RNA-mediated gene regulatory pathways focused on the cytoplasmic functions of such pathways, identifying roles for Argonaute/small RNA pathways in modulating chromatin and organizing the genome has become a topic of intense research in recent years. Nuclear regulatory mechanisms for Argonaute/small RNA pathways appear to be widespread, in organisms ranging from plants to fission yeast, Caenorhabditis elegans to humans. As the effectors of small RNA-mediated gene regulatory pathways, Argonaute proteins guide the chromatin-directed activities of these pathways. Of particular interest is the C. elegans Argonaute, chromosome segregation and RNAi deficient (CSR-1), which has been implicated in such diverse functions as organizing the holocentromeres of worm chromosomes, modulating germline chromatin, protecting the genome from foreign nucleic acid, regulating histone levels, executing RNAi, and inhibiting translation in conjunction with Pumilio proteins. CSR-1 interacts with small RNAs known as 22G-RNAs, which have complementarity to 25 % of the protein coding genes. This peculiar Argonaute is the only essential C. elegans Argonaute out of 24 family members in total. Here, we summarize the current understanding of CSR-1 functions in the worm, with emphasis on the chromatin-directed activities of this ever-intriguing Argonaute.

一个多任务的阿尔戈内特:探索秀丽隐杆线虫CSR-1的许多方面。
虽然对小RNA介导的基因调控途径的初步研究主要集中在这些途径的细胞质功能上,但鉴定Argonaute/小RNA途径在调节染色质和组织基因组中的作用已成为近年来研究的热点。从植物到裂变酵母,从秀丽隐杆线虫到人类,Argonaute/小RNA途径的核调控机制似乎是广泛存在的。Argonaute蛋白作为小rna介导的基因调控通路的效应器,引导染色质导向的这些通路的活性。特别令人感兴趣的是秀丽隐杆线虫,染色体分离和RNAi缺陷(CSR-1),它涉及多种功能,如组织蠕虫染色体的全新中心粒,调节种系染色质,保护基因组免受外来核酸的侵害,调节组蛋白水平,执行RNAi,以及抑制与Pumilio蛋白结合的翻译。CSR-1与被称为22g - rna的小rna相互作用,后者与25%的蛋白质编码基因具有互补性。这个奇特的阿尔戈内特是秀丽隐杆线虫在24个家族成员中唯一必需的阿尔戈内特。在这里,我们总结了目前对蠕虫中CSR-1功能的理解,重点介绍了这一令人着迷的Argonaute的染色质定向活动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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