Neuromedin B stimulates the hypothalamic–pituitary–gonadal axis in male rats

Regulatory Peptides Pub Date : 2013-11-10 DOI:10.1016/j.regpep.2013.10.002

C.K. Boughton , S.A. Patel , E.L. Thompson , M. Patterson , A.E. Curtis , A. Amin , K. Chen , M.A. Ghatei , S.R. Bloom , K.G. Murphy

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引用次数: 13

Abstract

Neuromedin B (NMB) is a highly conserved bombesin-related peptide found in mammals. NMB mRNA is detected in the central nervous system (CNS) and is highly expressed in the rat hypothalamus, in particular the medial preoptic area and the arcuate nucleus. The mammalian bombesin family of receptors consists of three closely related G protein coupled receptors, BB₁, BB₂ and BB₃. The BB₁ receptor subtype has the highest affinity for NMB.

NMB has well documented roles in the regulation of the thyroid axis and the stress axis in rats. However, there is little available data regarding the role of NMB in the regulation of the hypothalamic–pituitary–gonadal (HPG) axis. It is known that the NMB receptor is expressed in immortalised gonadotrophin releasing hormone (GnRH) releasing GT1-7 cells and murine forebrain GnRH neurons, and that anterior pituitary NMB-immunoreactivity is altered by changes in the sex steroid environment. The objective of these studies was thus to further investigate the effects of NMB on the HPG axis.

Intracerebroventricular (ICV) administration of NMB (10 nmol) to adult male rats significantly increased plasma luteinising hormone (LH) levels 30 min after injection (plasma LH ng/ml; saline 0.69 ± 0.07, 10 nmol NMB 1.33 ± 0.17, P < 0.01). In vitro, NMB stimulated GnRH release from hypothalamic explants from male rats and from hypothalamic GT1-7 cells. NMB had no significant effect on LH release from anterior pituitary explants from male rats, or from pituitary LβT2 cells in vitro.

These results suggest a previously unreported role for NMB in the stimulation of the HPG axis via hypothalamic GnRH. Further work is now required to determine the receptor mediating the effects of NMB on the reproductive axis and the physiological role of NMB in reproduction.

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神经素B刺激雄性大鼠下丘脑-垂体-性腺轴

神经蛋白B (Neuromedin B, NMB)是一种在哺乳动物中发现的高度保守的炸弹蛋白相关肽。NMB mRNA在中枢神经系统(CNS)中检测到，并在大鼠下丘脑，特别是内侧视前区和弓状核中高度表达。哺乳动物bombesin受体家族由三个密切相关的G蛋白偶联受体BB1、BB2和BB3组成。BB1受体亚型对NMB的亲和力最高。NMB在大鼠甲状腺轴和应激轴的调节中有很好的作用。然而，关于NMB在调节下丘脑-垂体-性腺(HPG)轴中的作用，几乎没有可用的数据。众所周知，NMB受体在永生化促性腺激素释放激素(GnRH)释放GT1-7细胞和小鼠前脑GnRH神经元中表达，并且垂体前叶NMB免疫反应性会因性类固醇环境的变化而改变。因此，这些研究的目的是进一步研究NMB对HPG轴的影响。成年雄性大鼠脑室内注射NMB (10 nmol)可显著提高注射后30 min血浆黄体生成素(LH)水平(血浆LH ng/ml;盐水0.69±0.07,10 nmol NMB 1.33±0.17,P & lt;0.01)。在体外，NMB刺激雄性大鼠下丘脑外植体和下丘脑GT1-7细胞释放GnRH。NMB对雄性大鼠垂体前叶外植体和垂体l - β t2细胞的LH释放无显著影响。这些结果表明，NMB在通过下丘脑GnRH刺激HPG轴中的作用以前未被报道。现在需要进一步的工作来确定介导NMB对生殖轴的影响的受体以及NMB在生殖中的生理作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

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审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.