Bruno Cesar Pereira, José Rodrigo Pauli, Lusânia Maria Greggi Antunes, Ellen Cristini de Freitas, Mara Ribeiro de Almeida, Vinícius de Paula Venâncio, Eduardo Rochete Ropelle, Claudio Teodoro de Souza, Dennys Esper Cintra, Marcelo Papoti, Adelino Sanchez Ramos da Silva
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引用次数: 0
Abstract
Background: The alkaline version of the single-cell gel (comet) assay is a useful method for quantifying DNA damage. Although some studies on chronic and acute effects of exercise on DNA damage measured by the comet assay have been performed, it is unknown if an aerobic training protocol with intensity, volume, and load clearly defined will improve performance without leading to peripheral blood cell DNA damage. In addition, the effects of overtraining on DNA damage are unknown. Therefore, this study aimed to examine the effects of aerobic training and overtraining on DNA damage in peripheral blood and skeletal muscle cells in Swiss mice. To examine possible changes in these parameters with oxidative stress, we measured reduced glutathione (GSH) levels in total blood, and GSH levels and lipid peroxidation in muscle samples.
Results: Performance evaluations (i.e., incremental load and exhaustive tests) showed significant intra and inter-group differences. The overtrained (OTR) group showed a significant increase in the percentage of DNA in the tail compared with the control (C) and trained (TR) groups. GSH levels were significantly lower in the OTR group than in the C and TR groups. The OTR group had significantly higher lipid peroxidation levels compared with the C and TR groups.
Conclusions: Aerobic and anaerobic performance parameters can be improved in training at maximal lactate steady state during 8 weeks without leading to DNA damage in peripheral blood and skeletal muscle cells or to oxidative stress in skeletal muscle cells. However, overtraining induced by downhill running training sessions is associated with DNA damage in peripheral blood and skeletal muscle cells, and with oxidative stress in skeletal muscle cells and total blood.
背景:碱性单细胞凝胶(彗星)测定法是一种量化 DNA 损伤的有用方法。虽然已经开展了一些关于慢性和急性运动对彗星测定法所测 DNA 损伤影响的研究,但目前尚不清楚明确规定强度、运动量和负荷的有氧训练方案是否能在提高运动成绩的同时不导致外周血细胞 DNA 损伤。此外,过度训练对 DNA 损伤的影响也不得而知。因此,本研究旨在研究有氧训练和过度训练对瑞士小鼠外周血和骨骼肌细胞DNA损伤的影响。为了研究这些参数可能随氧化应激而发生的变化,我们测量了总血液中还原型谷胱甘肽(GSH)的水平,以及肌肉样本中GSH水平和脂质过氧化反应:成绩评估(即增量负荷和耗竭测试)显示出显著的组内和组间差异。与对照组(C)和训练组(TR)相比,过度训练组(OTR)尾部 DNA 的百分比明显增加。OTR组的GSH水平明显低于C组和TR组。与 C 组和 TR 组相比,OTR 组的脂质过氧化水平明显更高:结论:在8周的最大乳酸稳态训练中,有氧和无氧性能参数均可得到改善,且不会导致外周血和骨骼肌细胞的DNA损伤或骨骼肌细胞的氧化应激。然而,下坡跑训练导致的过度训练与外周血和骨骼肌细胞中的DNA损伤以及骨骼肌细胞和总血液中的氧化应激有关。
BMC PhysiologyBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
9.60
自引率
0.00%
发文量
0
期刊介绍:
BMC Physiology is an open access journal publishing original peer-reviewed research articles in cellular, tissue-level, organismal, functional, and developmental aspects of physiological processes. BMC Physiology (ISSN 1472-6793) is indexed/tracked/covered by PubMed, MEDLINE, BIOSIS, CAS, EMBASE, Scopus, Zoological Record and Google Scholar.