{"title":"In vitro selection of BNA (LNA) aptamers.","authors":"Masayasu Kuwahara, Satoshi Obika","doi":"10.4161/adna.25786","DOIUrl":null,"url":null,"abstract":"<p><p>Recently, we achieved the first in vitro selection of 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) aptamers. High-affinity thrombin-binding aptamers (TBAs) were obtained from DNA-based libraries containing 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) in the 5'-primer region, using the method of capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX). Furthermore, a similar selection protocol could provide TBAs that contain B/L nucleotides in both primer and random regions. We review technical challenges involved in the generation of various BNA libraries using analogs of B/L nucleoside-5'-triphosphate and polymerase variants and also discuss applications of these libraries to the selection of BNA (LNA) aptamers, as well as future prospects for their therapeutic and diagnostic uses. </p>","PeriodicalId":8444,"journal":{"name":"Artificial DNA: PNA & XNA","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/adna.25786","citationCount":"56","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Artificial DNA: PNA & XNA","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4161/adna.25786","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 56
Abstract
Recently, we achieved the first in vitro selection of 2'-O,4'-C-methylene bridged/locked nucleic acid (2',4'-BNA/LNA) aptamers. High-affinity thrombin-binding aptamers (TBAs) were obtained from DNA-based libraries containing 2'-O,4'-C-methylene-bridged/linked bicyclic ribonucleotides (B/L nucleotides) in the 5'-primer region, using the method of capillary electrophoresis systematic evolution of ligands by exponential enrichment (CE-SELEX). Furthermore, a similar selection protocol could provide TBAs that contain B/L nucleotides in both primer and random regions. We review technical challenges involved in the generation of various BNA libraries using analogs of B/L nucleoside-5'-triphosphate and polymerase variants and also discuss applications of these libraries to the selection of BNA (LNA) aptamers, as well as future prospects for their therapeutic and diagnostic uses.
最近，我们首次实现了2'-O,4'- c -亚甲基桥接/锁定核酸(2'，4'-BNA/LNA)适体的体外筛选。利用毛细管电泳指数富集系统进化(CE-SELEX)方法，从5'引物区含有2'-O,4'- c -亚甲基桥接/连接双环核糖核苷酸(B/L核苷酸)的dna文库中获得了高亲和力的凝血酶结合适体(TBAs)。此外，类似的选择方案可以提供在引物和随机区域都含有B/L核苷酸的tba。我们回顾了使用B/L核苷-5'-三磷酸和聚合酶变体的类似物生成各种BNA文库所涉及的技术挑战，并讨论了这些文库在BNA (LNA)适体选择中的应用，以及它们在治疗和诊断方面的未来前景。
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