Gene expression profiling in peripheral white blood cells in response to the intake of food with different glycemic index using a DNA microarray.

Q Agricultural and Biological Sciences

Journal of Nutrigenetics and Nutrigenomics Pub Date : 2013-01-01 Epub Date: 2013-08-31 DOI:10.1159/000354247

Yuka Kawakami, Hisami Yamanaka-Okumura, Masae Sakuma, Yuka Mori, Chisaki Adachi, Yukie Matsumoto, Tadatoshi Sato, Hironori Yamamoto, Yutaka Taketani, Takafumi Katayama, Eiji Takeda

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引用次数: 15

Abstract

Background/aims: Transcriptomics technology in human nutrition intervention studies would allow for genome-wide screening of the effects of nutrients. We observed the time course of gene expression changes in peripheral white blood cells (WBC) to elucidate the metabolic changes in the postprandial state that are a reflection and a marker of whole body metabolic changes.

Methods: In a randomized crossover study, 7 healthy subjects consumed test meals of glucose (GL), white rice (WR) and rolled barley (BAR), each containing 75 g of available carbohydrate, and water (WAT). Blood glucose, insulin and nonesterified fatty acid concentrations, as well as the subjective levels of fullness and hunger were measured. Microarray analysis of the WBC and the real-time PCR were examined during 360 min after the intake of the test meals.

Results: The number of genes that changed more than 1.5-fold and the expression patterns in the time course were different between the GL, the WR and the BAR groups. Several genes involved in glycolysis and fatty acid β-oxidation were markedly changed after the intake of the GL, the WR and the BAR; however, these genes did not change at any time point in the WAT.

Conclusions: Gene expression profiling in the WBC can reflect food-related metabolic changes, even in the postprandial state.

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外周白细胞对摄入不同血糖指数食物的基因表达谱分析

背景/目的:人类营养干预研究中的转录组学技术将允许全基因组筛选营养物质的影响。我们通过观察外周白细胞(WBC)基因表达变化的时间过程来阐明餐后状态下的代谢变化是全身代谢变化的反映和标志。方法:在一项随机交叉研究中，7名健康受试者食用葡萄糖(GL)、白米(WR)和大麦(BAR)试验餐，每餐含有75 g有效碳水化合物和水(WAT)。测量血糖、胰岛素和非酯化脂肪酸浓度，以及饱腹感和饥饿感的主观水平。在进食试验餐后360分钟内进行白细胞微阵列分析和实时PCR检测。结果:GL组、WR组和BAR组在时间过程中改变1.5倍以上的基因数量和表达模式存在差异。摄入GL、WR和BAR后，参与糖酵解和脂肪酸β-氧化的几个基因发生了显著变化;然而，这些基因在WAT的任何时间点都没有改变。结论:白细胞中的基因表达谱可以反映食物相关的代谢变化，甚至在餐后状态下也是如此。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Nutrigenetics and Nutrigenomics GENETICS & HEREDITY-NUTRITION & DIETETICS

CiteScore

1.86

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The emerging field of nutrigenetics and nutrigenomics is rapidly gaining importance, and this new international journal has been established to meet the needs of the investigators for a high-quality platform for their research. Endorsed by the recently founded "International Society of Nutrigenetics/Nutrigenomics", the ‘Journal of Nutrigenetics and Nutrigenomics’ welcomes contributions not only investigating the role of genetic variation in response to diet and that of nutrients in the regulation of gene expression, but is also open for articles covering all aspects of gene-environment interactions in the determination of health and disease.