Solexa sequencing of microRNAs on chromium metabolism in broiler chicks.

Q Agricultural and Biological Sciences

Journal of Nutrigenetics and Nutrigenomics Pub Date : 2013-01-01 Epub Date: 2013-08-15 DOI:10.1159/000353703

Y Z Pan, S G Wu, H C Dai, H J Zhang, H Y Yue, G H Qi

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引用次数: 13

Abstract

Aim: The aim of this study was to determine the effect of chromium picolinate (CrPic) on the differential expression of the known microRNAs (miRNAs) in broiler skeletal muscle.

Methods and results: A total of 288 1-day-old male Arbor Acres broilers were randomly assigned to one of four dietary treatments supplemented with 0, 0.4, 2.0, or 10.0 mg·kg(-1) CrPic, respectively. Dietary CrPic supplementation at 10.0 mg·kg(-1) increased the average daily feed intake in broilers (p < 0.05). On day 42, the serum total protein level was highest in animals treated with 2.0 mg·kg(-1) (p < 0.05) and 10.0 mg·kg(-1) CrPic (p < 0.05). Dietary supplementation with 10.0 mg·kg(-1) CrPic decreased the levels of serum glucose (p < 0.05) on day 42 and of serum triglyceride (p < 0.05) on days 21 and 42. To further identify miRNAs from broiler skeletal muscles, we sequenced two small RNA libraries using the Solexa sequencing approach, and 57 miRNAs were found to be significantly differentially expressed (p < 0.05). Among them, 6 upregulated and 2 downregulated miRNAs were validated by real-time qPCR (p < 0.05).

Conclusions: The results of the present study provide a valuable clue regarding the role of miRNA target genes in the mechanism of the dietary CrPic effect on protein synthesis in skeletal muscles of broilers.

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肉仔鸡铬代谢microrna的Solexa测序。

目的:研究吡啶甲酸铬(CrPic)对肉鸡骨骼肌中已知microRNAs (miRNAs)差异表达的影响。方法与结果:288只1日龄爱拔益加肉仔鸡，随机分为4组，分别添加0、0.4、2.0和10.0 mg·kg(-1) CrPic。饲粮中添加10.0 mg·kg(-1)的CrPic可提高肉仔鸡平均日采食量(p < 0.05)。第42天，2.0 mg·kg(-1)和10.0 mg·kg(-1) CrPic组血清总蛋白水平最高(p < 0.05)。饲粮中添加10.0 mg·kg(-1) CrPic降低了第42天的血清葡萄糖水平(p < 0.05)和第21天和第42天的血清甘油三酯水平(p < 0.05)。为了进一步鉴定肉鸡骨骼肌的mirna，我们使用Solexa测序方法对两个小RNA文库进行了测序，发现57个mirna表达有显著差异(p < 0.05)。其中6个上调mirna和2个下调mirna通过real-time qPCR验证(p < 0.05)。结论:本研究结果为miRNA靶基因在饲粮中添加CrPic对肉鸡骨骼肌蛋白质合成影响机制中的作用提供了有价值的线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Nutrigenetics and Nutrigenomics GENETICS & HEREDITY-NUTRITION & DIETETICS

CiteScore

1.86

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The emerging field of nutrigenetics and nutrigenomics is rapidly gaining importance, and this new international journal has been established to meet the needs of the investigators for a high-quality platform for their research. Endorsed by the recently founded "International Society of Nutrigenetics/Nutrigenomics", the ‘Journal of Nutrigenetics and Nutrigenomics’ welcomes contributions not only investigating the role of genetic variation in response to diet and that of nutrients in the regulation of gene expression, but is also open for articles covering all aspects of gene-environment interactions in the determination of health and disease.