Comparison of immunomodulatory functions of three peptides from the chicken bursa of Fabricius

Regulatory Peptides Pub Date : 2013-09-10 DOI:10.1016/j.regpep.2013.07.007

Xiao-Dong Liu , Bin Zhou , Rui-Bing Cao , Xiu-Li Feng , Xin-Feng Li , Pu-Yan Chen

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引用次数: 11

Abstract

The bursa of Fabricius (BF) is the acknowledged central humoral immune organ unique to birds which plays important roles in B cell development and antibody production. Little information on immunomodulatory functions of BF is reported, except for several reported active bursal-derived peptides. Three peptides were identified and characterized from BF through RP-HPLC and MADIL-TOF methods. They are named as bursal peptide (BP)-I, BP-II, BP-III. These peptides promoted CFU pre-B formation and decreased PU.1 expression. The different immunomodulatory activities of these three bursal peptides on antibody and cytokine productions were verified by the immunization comparative experiment. The results showed the three bursal peptides enhanced AIV-specific antibody and cytokine production, T-cell immunophenotyping at reachable concentrations. These results indicate the important orientations for the comprehensive understanding of the humoral central immune system, and provide a novel insight on new experimental reagents for immuno-adjuvant or immunopharmacological.

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鸡法氏囊3种多肽免疫调节功能的比较

法氏囊(BF)是公认的鸟类特有的中枢体液免疫器官，在B细胞发育和抗体产生中起着重要作用。除了几种有活性的法氏囊衍生肽外，关于BF免疫调节功能的报道很少。通过反相高效液相色谱法和MADIL-TOF法对BF中3个多肽进行了鉴定和表征。它们被命名为法氏囊肽(BP)- 1、BP- ii、BP- iii。这些肽促进CFU前b的形成，降低PU.1的表达。通过免疫对比实验验证了这三种法氏囊肽对抗体和细胞因子产生的不同免疫调节活性。结果表明，三种法氏囊肽在可达到的浓度下增强了aiv特异性抗体和细胞因子的产生，t细胞免疫表型。这些结果为全面认识体液中枢免疫系统提供了重要方向，并为免疫佐剂或免疫药理学提供了新的实验试剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

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审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.