Ectopic expression of ghrelin affects gastric H+–K+-ATPase activity and expression of GHR/IGF-1 system in weaned mice

Regulatory Peptides Pub Date : 2013-09-10 DOI:10.1016/j.regpep.2013.06.002

G.M. Du , M.J. Liu , N. Parvizi , R.Q. Zhao

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引用次数: 4

Abstract

Objectives

Ghrelin has been implicated in the regulation of gastric growth and functional development, but it is yet to be determined whether and how ghrelin over-expression may modify gastric growth, gastric acid secretion and mRNA expression of other gastric endocrine hormones. 25-day-old mice were injected intramuscularly with vacant plasmid (VP) or recombinant plasmid expressing secretory ghrelin at the doses of 50 μg (LG) and 100 μg (HG).

Results

Expression of ghrelin mRNA was detected in muscles 15 days post-injection, being most abundant in HG mice. In accordance with the ghrelin expression, gastric weight increased (P < 0.05) in HG mice, compared with VP control group. Significant increase of gastric mucosa H⁺–K⁺-ATPase mRNA expression was detected in HG mice compared to VP control group (P < 0.05). Compared with VP mice, gastric somatostatin (SS) mRNA expression decreased in LG and HG mice (P < 0.05), while gastric gastrin expression had no significant difference.

Conclusions

I.M. injection of plasmid encoding ghrelin improved gastric growth and gastric acid secretion with decreased SS mRNA in weaned mice.

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胃饥饿素的异位表达影响断奶小鼠胃H+ -K +- atp酶活性和GHR/IGF-1系统的表达

目的胃饥饿素(ghrelin)参与胃生长和功能发育的调控，但其过表达是否以及如何影响胃生长、胃酸分泌和其他胃内分泌激素mRNA的表达尚不清楚。分别以50 μg (LG)和100 μg (HG)的剂量给25日龄小鼠肌肉注射表达分泌胃饥饿素的空质粒(VP)或重组质粒。结果ghrelin mRNA在注射后15 d的肌肉中有表达，在HG小鼠中表达最多。胃饥饿素的表达与胃重升高的趋势一致(P <0.05)，与VP对照组比较。与VP对照组相比，HG小鼠胃黏膜H+ -K +- atp酶mRNA表达显著升高(P <0.05)。与VP小鼠相比，LG和HG小鼠胃生长抑素(SS) mRNA表达降低(P <0.05)，而胃泌素的表达差异无统计学意义。注射编码胃饥饿素的质粒促进断奶小鼠胃生长和胃酸分泌，并降低SS mRNA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.