An Effort to Isolate Mycobacterium bovis from Environmental Substrates during Investigations of Bovine Tuberculosis Transmission Sites (Cattle Farms and Wildlife Areas) in Michigan, USA.

ISRN veterinary science Pub Date : 2011-09-22 Print Date: 2011-01-01 DOI:10.5402/2011/787181

Amanda E Fine, Daniel J O'Brien, Scott R Winterstein, John B Kaneene

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引用次数: 17

Abstract

Deer movements on cattle farms, wildlife feeding, and livestock management practices in Michigan are thought to create opportunities for indirect transmission of Mycobacterium bovis via environmental substrates. To confirm the presence of viable M. bovis in the environment, substrates were collected from 13 farms with culture-confirmed M. bovis in cattle and 5 sites with high prevalence of M. bovis in free-ranging deer. None of the samples processed for mycobacterial culture were positive for M. bovis. Agent, host, and landscape-level factors decrease the probability of detecting M. bovis in the environment using conventional mycobacterial culture. Molecular techniques that increase the probability of M. bovis detection in environmental substrates should be applied to known sites of M. bovis transmission in Michigan. In the interim, epidemiological investigations informed by experimental studies will be most effective in characterizing M. bovis persistence in the environment and its role in the indirect interspecies transmission of M. bovis.

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在美国密歇根州牛结核病传播点(牛场和野生动物区)调查中从环境底物中分离牛分枝杆菌的努力。

密歇根州养牛场的鹿活动、野生动物喂养和牲畜管理实践被认为为牛分枝杆菌通过环境基质间接传播创造了机会。为了确认环境中存在活的牛分枝杆菌，从13个牛场和5个自由放养鹿中牛分枝杆菌高流行点收集了底物。分枝杆菌培养处理的样品均未对牛分枝杆菌呈阳性反应。制剂、宿主和景观水平因素降低了使用常规分枝杆菌培养在环境中检测牛分枝杆菌的概率。提高环境底物中牛支原体检测概率的分子技术应应用于密歇根州牛支原体传播的已知地点。在此期间，基于实验研究的流行病学调查将最有效地描述牛支原体在环境中的持久性及其在牛支原体间接种间传播中的作用。

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ISRN veterinary science

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