Measurement of Horse Allergen (Equ cx) in Schools.

ISRN allergy Pub Date : 2012-01-04 Print Date: 2011-01-01 DOI:10.5402/2011/574258
Anne-Sophie Merritt, Gunnel Emenius, Lena Elfman, Greta Smedje
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Abstract

Background. The presence of horse allergen in public places is not well-known, unlike for instance cat and dog allergens, which have been studied extensively. The aim was to investigate the presence of horse allergen in schools and to what extent the influence of number of children with regular horse contact have on indoor allergen levels. Methods. Petri dishes were used to collect airborne dust samples during one week in classrooms. In some cases, vacuumed dust samples were also collected. All samples were extracted, frozen and analysed for Equ cx content shortly after sampling, and some were re-analysed six years later with a more sensitive ELISA assay. Results. Horse allergen levels were significantly higher in classrooms, in which many children had horse contact, regardless of sampling method. Allergen levels in extracts from Petri dish samples, which had been kept frozen, dropped about 53% over a six-year period. Conclusion. Horse allergen was present in classrooms and levels were higher in classrooms where many children had regular horse contact in their leisure time. This suggests that transfer of allergens takes place via contaminated clothing. Measures should be taken to minimize possible transfer and deposition of allergens in pet-free environments, such as schools.

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测量学校中的马过敏原 (Equ cx)。
背景。马过敏原在公共场所的存在并不为人所知,不像猫和狗等过敏原已被广泛研究。我们的目的是调查学校中是否存在马过敏原,以及经常接触马的儿童数量对室内过敏原水平的影响程度。研究方法使用培养皿在教室中收集一周的空气灰尘样本。在某些情况下,还收集了吸尘样本。所有样本均在采样后不久提取、冷冻并分析 Equ cx 含量,其中一些样本在六年后使用灵敏度更高的 ELISA 分析法重新进行分析。分析结果无论采用哪种采样方法,马过敏原在教室中的含量都明显较高,因为教室中有很多孩子接触过马。冷冻保存的培养皿样本提取物中的过敏原水平在六年内下降了约 53%。结论马过敏原存在于教室中,而在许多儿童在课余时间经常接触马的教室中,马过敏原的含量更高。这表明过敏原是通过受污染的衣物传播的。应采取措施尽量减少过敏原在学校等无宠物环境中的转移和沉积。
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