Structural and functional based identification of the bean (Phaseolus) microRNAs and their targets from expressed sequence tags.

Journal of structural and functional genomics Pub Date : 2013-03-01 Epub Date: 2013-04-19 DOI:10.1007/s10969-013-9152-z

Muhammad Younas Khan Barozai, Muhammad Din, Iftikhar Ahmed Baloch

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引用次数: 19

Abstract

MicroRNAs (miRNAs) are small, 18-26 nucleotides long, non-coding RNAs that play role in post-transcriptional gene regulation. Many of these are evolutionarily conserved. This suggests a powerful approach to predict new miRNAs in other species. In this research, structural and functional approaches were combined to make computational prediction of potential miRNAs and their targets in Bean (Phaseolus). Total 55 novel miRNAs were detected from 38 miRNAs families in Bean (Phaseolus). These families are; miR156, 160, 164, 168, 170, 171, 172, 319, 393, 396, 397, 398, 408, 414, 438, 444, 535, 1310, 1424, 1426, 1848, 1860, 1863, 2055, 2091, 2093, 2094, 2102, 2103, 2105, 2864, 2866, 2925, 2926, 4221, 4245, 4246 and 4250. In the 55 putative miRNAs; 28 miRNAs belong to Phaseolus acutifolius, 23 to Phaseolus vulgaris, 4 to Phaseolus coccineus. All the mature miRNAs reside in the stem portion of the hairpin structures. Total 146 potential protein targets were predicted for these miRNAs.

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基于结构和功能的大豆(Phaseolus) microrna及其靶标的序列标记鉴定。

MicroRNAs (miRNAs)是一种小的，长度为18-26个核苷酸的非编码rna，在转录后基因调控中发挥作用。其中许多是进化上保守的。这为预测其他物种的新mirna提供了一种强有力的方法。本研究将结构和功能相结合的方法对Bean (Phaseolus)中潜在的mirna及其靶点进行了计算预测。从38个mirna家族中共检测到55个新的mirna。这些家庭是;miR156、160、164、168、170、171、172、319、393、396、397、398、408、414、438、444、535、1310、1424、1426、1848、1860、1863、2055、2091、2093、2094、2102、2103、2105、2864、2866、2925、2926、4221、4245、4246、4250。在55个推测的mirna中;28个mirna属于尖头Phaseolus, 23个属于Phaseolus vulgaris, 4个属于Phaseolus coccineus。所有成熟的mirna都位于发夹结构的茎部。这些mirna共预测了146个潜在的蛋白靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of structural and functional genomics

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