[Design and implementation of a molecular method for influenza A virus (H1N1) in Cuba].

Q4 Medicine

Revista Cubana de Medicina Tropical Pub Date : 2011-01-01

Odalys Valdés Ramírez, Alexander Piñón Ramos, Belsy Acosta Herrera, Clara Savón Valdés, Grehete González Muñoz, Amely Arencibia García, Elías Guilarte García, Guelsys González Báez, Suset Oropeza Fernández, Bárbara Hernández Espinosa, Angel Goyenechea Hernández

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引用次数: 0

Abstract

Introduction: From March through April of 2009, Mexico notified outbreaks of respiratory illness, due to a new influenza virus of swine origin, which spread over rapidly via human-to-human transmission. The molecular methods currently in use were not suitable because the genome composition based on gene segments of swine, avian and human origin was quite different from the influenza A virus (H1N1) circulating at that time.

Objective: Based on the published sequences, a set of specific primers for the HA gene was designed to evaluate a new RT-PCR assay.

Methods: The RT-PCR assay processed 3 197 clinical samples from suspected cases of pandemic influenza A (H1N1) infection.

Results: The novel optimized method obtained a 262 pb segment, without unspecific reactions. The new method proved to be useful in the diagnosis and subtyping of pandemic HINI influenza virus. The amplified product was verified by nucleotide sequencing, thus confirming the virus.

Conclusions: The introduction of this new assay for the laboratory surveillance of influenza virus strengthens the diagnostic capacity of the National Reference Laboratory.

本刊更多论文

[在古巴设计和实施a型流感病毒(H1N1)的分子方法]。

从2009年3月到4月，墨西哥通报了由一种新型猪源流感病毒引起的呼吸道疾病暴发，这种病毒通过人与人之间的传播迅速传播。由于基于猪、禽和人源基因片段的基因组组成与当时流行的甲型流感病毒(H1N1)有很大不同，目前使用的分子方法并不适用。目的:基于已发表的HA基因序列，设计一套特异性引物，以评价一种新的RT-PCR检测方法。方法:采用RT-PCR方法对疑似甲型H1N1流感病例临床标本3 197份进行处理。结果:优化后的方法得到262 pb的片段，无非特异性反应。该方法可用于流感大流行病毒的诊断和分型。扩增产物经核苷酸测序证实为病毒。结论:采用这种用于流感病毒实验室监测的新检测方法加强了国家参考实验室的诊断能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Cubana de Medicina Tropical Medicine-Infectious Diseases

CiteScore

0.30

自引率

0.00%

发文量

期刊介绍： La Revista Cubana de Medicina Tropical tiene la misión de publicar artículos científicos especializados en medicina tropical, microbiología, parasitología, epidemiología y otras especialidades afines. Se distribuye directamente por el editor a los suscriptores en formato impreso (ISSN 0375-0760). Está dirigida a profesionales y técnicos en el campo de la medicina tropical, microbiología, parasitología y epidemiología. Recibe contribuciones en idioma español, inglés y portugués sin distinción en el país de procedencia.