Effects of felodipine combined with puerarin on ACE2–Ang (1–7)–Mas axis in renovascular hypertensive rat

Regulatory Peptides Pub Date : 2013-06-10 DOI:10.1016/j.regpep.2013.03.005

Song Bai, Zheng-Gui Huang, Li Chen, Jiang-Tao Wang, Bo-Ping Ding

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引用次数: 16

Abstract

This study aimed to investigate the effect of combination of felodipine + puerarin on ACE2–Ang (1–7)–Mas axis, and to explore the protective effect of the combination against kidney in renovascular hypertensive rats. Goldblatt rats were randomly divided into 5 groups as follows: 4 groups which were treated with felodipine (Felo), puerarin (Pue), Felo + Pue, and Felo + captopril (Cap), respectively, and a control group of animals that were administrated with distilled water. Contents of Ang II and Ang (1–7) in renal tissues were determined by ELISA kit. The mRNA expression of ACE2/Mas and ACE/AT₁ in kidneys was analyzed by RT-PCR. After 8 weeks of treatment, compared with Goldblatt group, Felo + Pue reduced SBP, DBP and HR (p < 0.01 or p < 0.05), ameliorated renal interstitial fibrosis, decreased the level of Ang II and increased that of Ang (1–7), upregulated mRNA expression of ACE2 and Mas, decreased that of ACE and AT₁, and downregulated protein expression of TGF-β₁ in kidneys (p < 0.01). Compared with Felo group, Felo + Pue decreased DBP and HR more markedly, attenuated fibrosis, decreased Ang II levels and increased those of Ang (1–7), upregulated mRNA expression of ACE2 in bilateral kidneys and that of Mas in ischemic kidney, downregulated that of ACE in bilateral kidneys and that of AT₁ in ischemic kidney, and decreased expression of TGF-β₁ protein significantly. In a word, a combination of Felo + Pue has a more efficient therapeutic effect on DBP and HR, and contributes to a better protection against renal interstitial fibrosis.

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非洛地平联合葛根素对肾血管性高血压大鼠ACE2-Ang (1-7) -Mas轴的影响

本研究旨在探讨非洛地平+葛根素联合用药对肾血管性高血压大鼠ACE2-Ang (1-7) -Mas轴的影响，探讨联合用药对肾脏的保护作用。将Goldblatt大鼠随机分为5组:4组分别给予非洛地平(Felo)、葛根素(Pue)、Felo + Pue、Felo +卡托普利(Cap)，对照组给予蒸馏水。采用酶联免疫吸附测定法测定肾组织中Angⅱ和Ang(1-7)的含量。采用RT-PCR方法分析肾脏组织中ACE2/Mas和ACE/AT1 mRNA的表达。治疗8周后，与Goldblatt组比较，Felo + Pue组降低收缩压、舒张压和心率(p <0.01或p <0.05)，改善肾间质纤维化，降低Ang II水平，升高Ang水平(1-7)，上调肾脏ACE2和Mas mRNA表达，降低ACE和AT1 mRNA表达，下调TGF-β1蛋白表达(p <0.01)。与Felo组相比，Felo + Pue更显著降低舒张压和心率，减轻纤维化，降低Ang II水平并升高Ang(1-7)，上调双侧肾脏ACE2和缺血肾脏Mas mRNA表达，下调双侧肾脏ACE和缺血肾脏AT1 mRNA表达，显著降低TGF-β1蛋白表达。综上所述，Felo + Pue联合使用对DBP和HR的治疗效果更有效，对肾间质纤维化有更好的保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

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审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.