Spatial and temporal MRI profile of ischemic tissue after the acute stages of a permanent mouse model of stroke.

Pub Date : 2013-01-01 Epub Date: 2013-02-01 DOI:10.2174/1874440001307010004
A Bogaert-Buchmann, M Poittevin, C Po, D Dupont, C Sebrié, Y Tomita, A Trandinh, J Seylaz, E Pinard, P Méric, N Kubis, B Gillet
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引用次数: 6

Abstract

Object: To characterize the progression of injured tissue resulting from a permanent focal cerebral ischemia after the acute phase, Magnetic Resonance Imaging (MRI) monitoring was performed on adult male C57BL/6J mice in the subacute stages, and correlated to histological analyses.

Material and methods: Lesions were induced by electrocoagulation of the middle cerebral artery. Serial MRI measurements and weighted-images (T2, T1, T2* and Diffusion Tensor Imaging) were performed on a 9.4T scanner. Histological data (Cresyl-Violet staining and laminin-, Iba1- and GFAP-immunostainings) were obtained 1 and 2 weeks after the stroke.

Results: Two days after stroke, tissues assumed to correspond to the infarct core, were detected as a hyperintensity signal area in T2-weighted images. One week later, low-intensity signal areas appeared. Longitudinal MRI study showed that these areas remained present over the following week, and was mainly linked to a drop of the T2 relaxation time value in the corresponding tissues. Correlation with histological data and immuno-histochemistry showed that these areas corresponded to microglial cells.

Conclusion: The present data provide, for the first time detailed MRI parameters of microglial cells dynamics, allowing its non-invasive monitoring during the chronic stages of a stroke. This could be particularly interesting in regards to emerging anti-inflammatory stroke therapies.

Abstract Image

Abstract Image

Abstract Image

永久性脑卒中小鼠模型急性期后缺血组织的时空MRI特征。
目的:对成年雄性C57BL/6J小鼠进行亚急性期核磁共振成像(MRI)监测,并与组织学分析相关联,探讨永久性局灶性脑缺血急性期后损伤组织的进展。材料和方法:采用脑中动脉电凝法诱导病变。在9.4T扫描仪上进行系列MRI测量和加权图像(T2、T1、T2*和弥散张量成像)。脑卒中后1周和2周获得组织学数据(甲酰紫染色、层粘连蛋白、Iba1和gfap免疫染色)。结果:脑卒中后2天,假定与梗死核心相对应的组织在t2加权图像中被检测到高信号区。一周后,低强度信号区出现。纵向MRI研究表明,这些区域在接下来的一周内仍然存在,并且主要与相应组织的T2松弛时间值下降有关。与组织学数据和免疫组织化学的相关性表明,这些区域对应于小胶质细胞。结论:目前的数据首次提供了小胶质细胞动力学的详细MRI参数,允许其在中风慢性阶段进行无创监测。这对于新兴的抗炎中风疗法来说可能特别有趣。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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