Effects of exogenous vasoactive intestinal peptide on mesenteric lymph pathway during early intestinal ischemia–reperfusion injury in rats

Regulatory Peptides Pub Date : 2013-09-10 DOI:10.1016/j.regpep.2013.06.014

Hui Yang , Yu Jin , Chun H. Wang , Cheng W. Tang

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引用次数: 3

Abstract

Mesenteric lymph pathway serves as the primary route by which gut injury leads to systemic inflammation and distant organ injury. The inflammation of the intestinal tract is partially mediated by vasoactive intestinal peptide (VIP). Therefore, the aim of this study was to test whether exogenous VIP affects mesenteric lymph pathway during early intestinal ischemia–reperfusion (IIR) injury. Rats were randomized into control, control + VIP, IIR and IIR + VIP groups. The observation of mesenteric lymph flow was carried out by cannulation of mesenteric lymphatics. The distribution of in vivo lymphocyte trafficking was performed by ⁵¹Cr labeled lymphocytes and was measured by γ-counter. Endotoxin concentration was assayed using the limulus test kit and TNF-α level was detected by ELISA. When IIR injury treated with VIP, the volumes of lymph flow increased by 80%, which caused the number of lymphocytes exiting in mesenteric lymphatic increased by 50% while the proportion of ⁵¹Cr-lymphocytes in Peyer's patches, intestinal effector tissues, mesenteric nodes, large intestine, stomach decreased by 58%, 51%, 58%, 63%, 64% respectively at the 6th h after reperfusion following intestinal ischemia. Meanwhile, endotoxin and TNF-α levels in intestinal lymph decreased by 51% and 83%. These results suggest that exogenous VIP ameliorates IIR induced splanchnic organ damage via inhibition of toxic mediators reaching systemic circulation and reinforcement of the effective immune responses in gut-associated lymphoid tissues (GALT).

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外源性血管活性肠肽对早期肠缺血再灌注损伤大鼠肠系膜淋巴通路的影响

肠系膜淋巴通路是肠道损伤导致全身炎症和远端脏器损伤的主要途径。血管活性肠肽(VIP)在一定程度上介导了肠道炎症的发生。因此，本研究的目的是检测外源性VIP在早期肠缺血再灌注(IIR)损伤中是否影响肠系膜淋巴通路。将大鼠随机分为对照组、对照组+ VIP组、IIR组和IIR + VIP组。采用肠系膜淋巴管插管法观察肠系膜淋巴流。用51Cr标记淋巴细胞进行体内淋巴细胞运输分布，并用γ-计数器测定。采用鲎试剂检测内毒素浓度，ELISA法检测TNF-α水平。VIP处理IIR损伤时，淋巴流量增加80%，导致肠系膜淋巴细胞数量增加50%，而在肠缺血再灌注后第6 h, Peyer’s patches、肠效应组织、肠系膜淋巴结、大肠、胃中51cr -淋巴细胞的比例分别减少58%、51%、58%、63%、64%。肠道淋巴组织内毒素和TNF-α水平分别下降51%和83%。这些结果表明，外源性VIP通过抑制到达体循环的毒性介质和增强肠道相关淋巴组织(GALT)的有效免疫反应，改善IIR诱导的内脏器官损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Regulatory Peptides 医学-内分泌学与代谢

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Regulatory Peptides provides a medium for the rapid publication of interdisciplinary studies on the physiology and pathology of peptides of the gut, endocrine and nervous systems which regulate cell or tissue function. Articles emphasizing these objectives may be based on either fundamental or clinical observations obtained through the disciplines of morphology, cytochemistry, biochemistry, physiology, pathology, pharmacology or psychology.