Genotype distribution of human papillomaviruses in Japanese women with abnormal cervical cytology.

The Open Virology Journal Pub Date : 2012-01-01 Epub Date: 2012-12-28 DOI:10.2174/1874357901206010277
Kazunari Kondo, Asami Uenoyama, Ryo Kitagawa, Hajime Tsunoda, Rika Kusumoto-Matsuo, Seiichiro Mori, Yoshiyuki Ishii, Takamasa Takeuchi, Tadahito Kanda, Iwao Kukimoto
{"title":"Genotype distribution of human papillomaviruses in Japanese women with abnormal cervical cytology.","authors":"Kazunari Kondo,&nbsp;Asami Uenoyama,&nbsp;Ryo Kitagawa,&nbsp;Hajime Tsunoda,&nbsp;Rika Kusumoto-Matsuo,&nbsp;Seiichiro Mori,&nbsp;Yoshiyuki Ishii,&nbsp;Takamasa Takeuchi,&nbsp;Tadahito Kanda,&nbsp;Iwao Kukimoto","doi":"10.2174/1874357901206010277","DOIUrl":null,"url":null,"abstract":"<p><p>We report the prevalence and genotype distribution of human papillomaviruses (HPVs) among Japanese women with abnormal cervical cytology using the PGMY-CHUV assay, one of PGMY-PCR-based lineblot assays that was validated and shown to be suitable for the detection of multiple HPV types in a specimen with minimum bias. Total DNA was extracted from cervical exfoliated cells collected from 326 outpatients with abnormal Pap smears. Overall, 307 specimens (94%) were HPV-positive, 30% of which contained multiple genotypes. The prevalence of HPV DNA was 83% (49/59 samples) in atypical squamous cells of undetermined significance (ASC-US); 91% (20/22 samples) in atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H); 97% (130/134 samples) in low-grade squamous intraepithelial lesion (LSIL); and 99% (85/86 samples) in high-grade squamous intraepithelial lesion (HSIL). Three most frequent HPV types detected in HSIL were HPV16 (36%), HPV52 (24%), and HPV58 (14%). Our results suggest that multiple HPV infections are more prevalent in Japanese women than previously reported, and confirm that HPV52 and 58 are more dominant in their cervical precancerous lesions when compared to those reported in Western countries.</p>","PeriodicalId":23111,"journal":{"name":"The Open Virology Journal","volume":"6 ","pages":"277-83"},"PeriodicalIF":0.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/22/TOVJ-6-277.PMC3547357.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Virology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874357901206010277","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2012/12/28 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

Abstract

We report the prevalence and genotype distribution of human papillomaviruses (HPVs) among Japanese women with abnormal cervical cytology using the PGMY-CHUV assay, one of PGMY-PCR-based lineblot assays that was validated and shown to be suitable for the detection of multiple HPV types in a specimen with minimum bias. Total DNA was extracted from cervical exfoliated cells collected from 326 outpatients with abnormal Pap smears. Overall, 307 specimens (94%) were HPV-positive, 30% of which contained multiple genotypes. The prevalence of HPV DNA was 83% (49/59 samples) in atypical squamous cells of undetermined significance (ASC-US); 91% (20/22 samples) in atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H); 97% (130/134 samples) in low-grade squamous intraepithelial lesion (LSIL); and 99% (85/86 samples) in high-grade squamous intraepithelial lesion (HSIL). Three most frequent HPV types detected in HSIL were HPV16 (36%), HPV52 (24%), and HPV58 (14%). Our results suggest that multiple HPV infections are more prevalent in Japanese women than previously reported, and confirm that HPV52 and 58 are more dominant in their cervical precancerous lesions when compared to those reported in Western countries.

Abstract Image

Abstract Image

Abstract Image

日本宫颈细胞学异常妇女中人乳头瘤病毒的基因型分布
我们报告了人类乳头瘤病毒(HPV)在宫颈细胞学异常的日本女性中的患病率和基因型分布,使用PGMY-CHUV检测,pgmy - pcr为基础的线印迹检测之一,被验证并证明适用于检测多种HPV类型的标本,偏差最小。从326例宫颈涂片检查异常的门诊患者采集的宫颈脱落细胞中提取总DNA。总体而言,307份标本(94%)为hpv阳性,其中30%含有多种基因型。在未确定意义的非典型鳞状细胞(ASC-US)中,HPV DNA的患病率为83%(49/59份样本);91%(20/22例)为非典型鳞状细胞,不能排除高级别鳞状上皮内病变(ASC-H);97%(130/134)为低级别鳞状上皮内病变(LSIL);99%(85/86例)为高度鳞状上皮内病变(HSIL)。在HSIL中最常见的三种HPV类型是HPV16(36%)、HPV52(24%)和HPV58(14%)。我们的研究结果表明,日本女性的多重HPV感染比以前报道的更为普遍,并且与西方国家的报道相比,证实了HPV52和58在宫颈癌前病变中更占优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信