{"title":"Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry.","authors":"Mehdi Taghdiri, Arash Mohamadipour-Taziyan","doi":"10.5402/2012/216068","DOIUrl":null,"url":null,"abstract":"<p><p>A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps. Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution. This attenuation was used to the microdetermination of dopamine by measurement of absorbance of the solid phase (Sephadex LH-20 gel) in a 1.0 mm cell at 625 nm. Dopamine could be determined in the concentration range of 0.4-1.6 μg mL(-1) (10-mL Sample volume) with a relative standard deviation (RSD) of 0.03% (n = 4). The detection limit was obtained, 0.26 μg mL(-1) (1.7 μM). The method was used for determination of dopamine in pharmaceutical injection sample and satisfactory result was obtained.</p>","PeriodicalId":14802,"journal":{"name":"ISRN Pharmaceutics","volume":" ","pages":"216068"},"PeriodicalIF":0.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/216068","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ISRN Pharmaceutics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5402/2012/216068","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2012/11/28 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
Abstract
A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps. Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution. This attenuation was used to the microdetermination of dopamine by measurement of absorbance of the solid phase (Sephadex LH-20 gel) in a 1.0 mm cell at 625 nm. Dopamine could be determined in the concentration range of 0.4-1.6 μg mL(-1) (10-mL Sample volume) with a relative standard deviation (RSD) of 0.03% (n = 4). The detection limit was obtained, 0.26 μg mL(-1) (1.7 μM). The method was used for determination of dopamine in pharmaceutical injection sample and satisfactory result was obtained.
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