Continuous X-ray diffractive field in protein nanocrystallography.

IF 1.8 4区材料科学

Acta Crystallographica Section A Pub Date : 2013-01-01 Epub Date: 2012-11-16 DOI:10.1107/S0108767312042535

Ruben A Dilanian, Victor A Streltsov, Harry M Quiney, Keith A Nugent

引用次数: 20

Abstract

The recent development of X-ray free-electron laser sources has created new opportunities for the structural analysis of protein nanocrystals. The extremely small sizes of the crystals, as well as imperfections of the crystal structure, result in an interference phenomenon in the diffraction pattern. With decreasing crystallite size the structural imperfections play a role in the formation of the diffraction pattern that is comparable in importance to the size effects and should be taken into account during the data analysis and structure reconstruction processes. There now exists a need to develop new methods of protein structure determination that do not depend on the availability of good-quality crystals and that can treat proteins under conditions close to the active form. This paper demonstrates an approach that is specifically tailored to nanocrystalline samples and offers a unique crystallographic solution.

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连续x射线衍射场在蛋白质纳米晶体学中的应用。

近年来x射线自由电子激光源的发展为蛋白质纳米晶体的结构分析创造了新的机会。晶体的极小尺寸以及晶体结构的缺陷导致了衍射图样中的干涉现象。随着晶粒尺寸的减小，结构缺陷在衍射图样的形成中起着与尺寸效应同等重要的作用，在数据分析和结构重建过程中应予以考虑。现在有必要开发新的蛋白质结构测定方法，这种方法不依赖于高质量晶体的可用性，并且可以在接近活性形式的条件下处理蛋白质。本文展示了一种专门针对纳米晶体样品的方法，并提供了一种独特的晶体学解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Crystallographica Section A 化学-晶体学

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： Acta Crystallographica Section A: Foundations and Advances publishes articles reporting advances in the theory and practice of all areas of crystallography in the broadest sense. As well as traditional crystallography, this includes nanocrystals, metacrystals, amorphous materials, quasicrystals, synchrotron and XFEL studies, coherent scattering, diffraction imaging, time-resolved studies and the structure of strain and defects in materials. The journal has two parts, a rapid-publication Advances section and the traditional Foundations section. Articles for the Advances section are of particularly high value and impact. They receive expedited treatment and may be highlighted by an accompanying scientific commentary article and a press release. Further details are given in the November 2013 Editorial. The central themes of the journal are, on the one hand, experimental and theoretical studies of the properties and arrangements of atoms, ions and molecules in condensed matter, periodic, quasiperiodic or amorphous, ideal or real, and, on the other, the theoretical and experimental aspects of the various methods to determine these properties and arrangements.