Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.

IF 0.9 4区 生物学
Nikolaus Goessweiner-Mohr, Christian Fercher, Mohammad Yaser Abajy, Elisabeth Grohmann, Walter Keller
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引用次数: 5

Abstract

Conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. The successful purification and crystallization of the putative transfer protein TraN from the G+ conjugative model plasmid pIP501 of Enterococcus faecalis are presented. Native crystals diffracted to 1.8 Å resolution on a synchrotron beamline. The crystals belonged to space group P2(1), with unit-cell parameters a=32.88, b=54.94, c=57.71 Å, β=91.89° and two molecules per asymmetric unit.

Abstract Image

Abstract Image

Abstract Image

革兰氏阳性结合质粒pIP501推定转移蛋白TraN的结晶和首次数据收集。
结合质粒转移是细菌间抗性和毒力基因传播的最重要途径。因此,携带结合质粒的细菌对人类健康,尤其是住院患者的健康构成了重大威胁。虽然已经获得了关于革兰氏阴性4型分泌系统的过程的详细信息,但对革兰氏阳性(G+)细菌的相应机制知之甚少。本文报道了从粪肠球菌G+偶联模型质粒pIP501中成功纯化和结晶假定的转移蛋白TraN。原生晶体衍射到1.8 Å分辨率。晶体属于空间群P2(1),晶胞参数a=32.88,b=54.94,c=57.71 Å,β=91.89°,每个不对称单元有两个分子。
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来源期刊
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审稿时长
2-4 weeks
期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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