Index of CD34+ Cells and Mononuclear Cells in the Bone Marrow of Spinal Cord Injury Patients of Different Age Groups: A Comparative Analysis.

Bone Marrow Research Pub Date : 2012-01-01 Epub Date: 2012-07-05 DOI:10.1155/2012/787414
Vidyasagar Devaprasad Dedeepiya, Yegneswara Yellury Rao, Gosalakkal A Jayakrishnan, Jutty K B C Parthiban, Subramani Baskar, Sadananda Rao Manjunath, Rajappa Senthilkumar, Samuel J K Abraham
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引用次数: 25

Abstract

Introduction. Recent evidence of safety and efficacy of Bone Marrow Mononuclear Cells (BMMNC) in spinal cord injury makes the Bone Marrow (BM) CD34+ percentage and the BMMNC count gain significance. The indices of BM that change with body mass index and aging in general population have been reported but seldom in Spinal Cord Injury (SCI) victims, whose parameters of relevance differ from general population. Herein, we report the indices of BMMNC in SCI victims. Materials and Methods. BMMNCs of 332 SCI patients were isolated under GMP protocols. Cell count by Trypan blue method and CD34+ cells by flow cytometry were documented and analysed across ages and gender. Results. The average BMMNC per ml in the age groups 0-20, 21-40, 41-60, and 61-80 years were 4.71, 4.03, 3.67, and 3.02 million and the CD34+ were 1.05%, 1.04%, 0.94%, and 0.93% respectively. The decline in CD34+ was sharp between 20-40 and 40-60 age groups. Females of reproductive age group had lesser CD34+. Conclusion. The BMMNC and CD34+ percentages decline with aging in SCI victims. Their lower values in females during reproductive age should be analysed for relevance to hormonal influence. This study offers reference values of BMMNC and CD34+ of SCI victims for successful clinical application.

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不同年龄组脊髓损伤患者骨髓中CD34+细胞和单核细胞指数的比较分析。
介绍。骨髓单个核细胞(BMMNC)治疗脊髓损伤的安全性和有效性的最新证据使得骨髓(BM) CD34+百分比和BMMNC计数具有重要意义。在一般人群中,BM指标随体重指数和年龄变化的报道较多,但在脊髓损伤(SCI)患者中报道较少,其相关参数与一般人群不同。在此,我们报告脊髓损伤患者的BMMNC指标。材料与方法。采用GMP方案分离332例SCI患者的bmmnc。台盼蓝法细胞计数和流式细胞术CD34+细胞计数记录并分析不同年龄和性别的差异。结果。0 ~ 20岁、21 ~ 40岁、41 ~ 60岁、61 ~ 80岁年龄组BMMNC / ml平均值分别为4.71、4.03、3.67、302万,CD34+分别为1.05%、1.04%、0.94%、0.93%。CD34+的下降在20-40岁和40-60岁年龄组之间明显。育龄女性CD34+较低。结论。脊髓损伤患者的BMMNC和CD34+百分比随着年龄的增长而下降。应分析育龄妇女的较低值与激素影响的关系。本研究为脊髓损伤患者BMMNC和CD34+的临床应用提供了参考价值。
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