Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro.

ISRN Pharmaceutics Pub Date : 2012-01-01 Epub Date: 2012-03-07 DOI:10.5402/2012/369270
Christian Jreyssaty, Qin Shi, Huijie Wang, Xingping Qiu, Françoise M Winnik, Xiaoling Zhang, Kerong Dai, Mohamed Benderdour, Julio C Fernandes
{"title":"Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro.","authors":"Christian Jreyssaty,&nbsp;Qin Shi,&nbsp;Huijie Wang,&nbsp;Xingping Qiu,&nbsp;Françoise M Winnik,&nbsp;Xiaoling Zhang,&nbsp;Kerong Dai,&nbsp;Mohamed Benderdour,&nbsp;Julio C Fernandes","doi":"10.5402/2012/369270","DOIUrl":null,"url":null,"abstract":"<p><p>Nonviral cationic polymers like chitosan can be combined with DNA to protect it from degradation. The chitosan is a biocompatible, biodegradable, nontoxic, and cheap polycationic polymer with low immunogenicity. The objective of this study was to synthesize and then assess different chitosan-DNA nanoparticles and to select the best ones for selective in vitro transfection in human epidermoid carcinoma (KB) cell lines. It revealed that different combinations of molecular weight, the presence or absence of folic acid ligand, and different plasmid DNA sizes can lead to nanoparticles with various diameters and diverse transfection efficiencies. The intracellular trafficking, nuclear uptake, and localization are also studied by confocal microscopy, which confirmed that DNA was delivered to cell nuclei to be expressed.</p>","PeriodicalId":14802,"journal":{"name":"ISRN Pharmaceutics","volume":" ","pages":"369270"},"PeriodicalIF":0.0000,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2012/369270","citationCount":"18","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ISRN Pharmaceutics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5402/2012/369270","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2012/3/7 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 18

Abstract

Nonviral cationic polymers like chitosan can be combined with DNA to protect it from degradation. The chitosan is a biocompatible, biodegradable, nontoxic, and cheap polycationic polymer with low immunogenicity. The objective of this study was to synthesize and then assess different chitosan-DNA nanoparticles and to select the best ones for selective in vitro transfection in human epidermoid carcinoma (KB) cell lines. It revealed that different combinations of molecular weight, the presence or absence of folic acid ligand, and different plasmid DNA sizes can lead to nanoparticles with various diameters and diverse transfection efficiencies. The intracellular trafficking, nuclear uptake, and localization are also studied by confocal microscopy, which confirmed that DNA was delivered to cell nuclei to be expressed.

Abstract Image

Abstract Image

Abstract Image

体外利用叶酸靶向壳聚糖- dna纳米颗粒进行有效的非病毒基因治疗。
壳聚糖等非病毒阳离子聚合物可以与DNA结合,防止DNA降解。壳聚糖是一种生物相容性、可生物降解、无毒、廉价的低免疫原性聚阳离子聚合物。本研究的目的是合成不同的壳聚糖- dna纳米颗粒并对其进行评价,并筛选出最佳的壳聚糖- dna纳米颗粒,用于体外选择性转染人表皮样癌(KB)细胞。结果表明,不同的分子量组合、叶酸配体的存在或不存在以及不同的质粒DNA大小可以导致不同直径和不同转染效率的纳米颗粒。通过共聚焦显微镜研究了细胞内运输、核摄取和定位,证实了DNA被传递到细胞核中进行表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信