Mannitol Reduces the Hydrostatic Pressure in the Proximal Tubule of the Isolated Blood-Perfused Rabbit Kidney during Hypoxic Stress and Improves Its Function.

Nephron Extra Pub Date : 2011-01-01 Epub Date: 2011-11-04 DOI:10.1159/000333478
Robbert Bipat, Paul Steels, Yves Cuypers, Jerry R Toelsie
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引用次数: 6

Abstract

Background/aims: Hypoxia may play a role in the development of renal failure in donated kidneys. In the present study, the effects of hypoxia on isolated blood-perfused rabbit kidneys were investigated and the effects of mannitol were explored, giving special attention to intratubular pressure.

Methods: Kidneys were perfused with their autologous blood during four 30-min periods (P1-P4). P1 was considered baseline function. In P2, hypoxia was induced either alone or with an infusion of mannitol (15 mg/min) during P2-P4. Reoxygenation was applied after P2. Proximal intratubular pressure was measured in all conditions.

Results: During hypoxia, renal blood flow doubled and restored immediately in P3. Urine flow stopped in P2, except in the series with mannitol, but gradually resumed in P3 and P4. Likewise, creatinine clearance recovered slightly (<25%) in P4, except for the series with mannitol, where it still could be measured in P2 and reached a value >50% of P1. Proximal intratubular pressure (mean ± SD) increased from 12 ± 5 in P1 to 24 ± 11 mm Hg during hypoxia and returned to 10 ± 6 mm Hg in P3. This increase was not observed with mannitol.

Conclusion: Cellular swelling might be responsible for the suppressed filtration during hypoxia and can be prevented by mannitol.

Abstract Image

Abstract Image

Abstract Image

甘露醇降低离体血灌注兔肾近端静水压力并改善其功能。
背景/目的:缺氧可能在捐赠肾脏肾功能衰竭的发展中起作用。在本研究中,我们研究了缺氧对离体血灌注兔肾脏的影响,并探讨了甘露醇的作用,特别关注了小管内压力。方法:在4个30min周期(p1 ~ p4)内灌注肾脏自体血。P1被认为是基线功能。P2组在P2- p4期间单独或联合输注甘露醇(15 mg/min)诱导缺氧。P2后再加氧。在所有情况下测量近端小管内压力。结果:缺氧时P3期肾血流量增加一倍并立即恢复。除甘露醇组外,尿流在P2期停止,但在P3和P4期逐渐恢复。同样,肌酐清除率略有恢复(P1的50%)。近端小管内压(平均±SD)从P1的12±5升高到缺氧时的24±11 mm Hg, P3时又恢复到10±6 mm Hg。甘露醇没有观察到这种增加。结论:细胞肿胀可能是缺氧时滤过抑制的原因,甘露醇可预防细胞肿胀。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊介绍: An open-access subjournal to Nephron. ''Nephron EXTRA'' publishes additional high-quality articles that cannot be published in the main journal ''Nephron'' due to space limitations.
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