Ganoderma lucidum reduces kainic acid-induced hippocampal neuronal damage via inflammatory cytokines and glial fibrillary acid protein expression.

A C Aguirre Moreno, V Campos Peña, J Villeda Hernández, I León Rivera, E Montiel Arcos
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Abstract

Ganoderma lucidum, a traditional Chinese medicine, has been shown to target the Central Nervous System. In this work we analyze whether G. lucidum, collected in Mexico, has a protective effect in the hippocampus of rats treated with kainic acid, a neurotoxin that causes seizures and neuronal loss. The aqueous extract of G. lucidum (10 mg/Kg, i.p.) was administered to rats 30 min before kainic acid injection (5 mg/Kg, i.p.). Animals that had received prior treatment with G. lucidum showed no tonic-clonic seizure activity. Histopathological analysis showed a significant decrease in neuronal loss and cellular alterations in the hippocampal CA3 region. Immunohistochemical analysis shows that when using G. lucidum in rats, there is less immunoreactivity for GFAP as well as TNF-alpha and IL-1beta in the CA3 region when compared with rats treated with kainic acid. Our results demonstrate that G. lucidum protects against kainic acid-induced alterations of hippocampal cells and expression of immunological markers in this model of excitotoxicity.

灵芝通过炎症细胞因子和胶质原纤维酸蛋白表达减少凯因酸诱导的海马神经元损伤。
灵芝,一种传统的中药,已经被证明是针对中枢神经系统的。在这项工作中,我们分析了在墨西哥采集的灵芝是否对用kainic酸治疗的大鼠的海马体有保护作用,kainic酸是一种导致癫痫发作和神经元丧失的神经毒素。大鼠在kainic酸(5 mg/Kg, i.p)注射前30 min给予灵芝水提物(10 mg/Kg, i.p)。先前接受过灵芝治疗的动物没有出现强直阵挛性发作活动。组织病理学分析显示海马CA3区神经元丢失和细胞改变显著减少。免疫组化分析显示,与kainic酸处理的大鼠相比,大鼠在使用灵芝时,对GFAP以及CA3区tnf - α和il -1 β的免疫反应性较低。我们的研究结果表明,在这种兴奋性毒性模型中,灵芝可以保护海马体细胞和免疫标记物的表达免受kainic酸诱导的改变。
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