Pharmacological evaluation of the immunomodulatory FL-6 in induced chronic hepatitis in Wistar rats.

U Osuna-Martinez, J A Reyes-Esparza, V l Petricevich, L Rodríguez-Fragoso
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Abstract

The aim of this study was to evaluate the pharmacological effect of FL-6, a new immunomodulatory drug, in chronic hepatitis immunologically induced in rats via porcine-serum (PS) administration. Thirty-two male Wistar rats (150 g) were divided into 4 experimental groups: (1) Control (PBS 0.5 ml 3-times per week for 8-week); (2) FL-6 (50 ng/kg 3-times per week for 4-week); (3) Hepatitis (PS 373 mg/kg twice per week for 8-week); and (4) Hepatitis + FL-6 (doses as above). Rats were sacrificed at the end of treatment. ALT, AST, ALP and gamma-GT activities, as well as IL-6 and IL-10 levels, were evaluated in serum samples. Glutathione and malondialdehyde were also analyzed. A morphological analysis of liver tissue was carried out. The hepatitis group showed an increase in ALT (1.44-fold), AST (1.28-fold), ALP (1.83-fold), gamma-GT (3.91-fold), IL-6 (2.6-fold) and IL-10 (7.1-fold) levels when compared with controls (p < 0.05). Histopathological analysis revealed an inflammatory response characterized by inflammatory infiltrates and liver damage, which was accompanied by a reduction of 74.8% in glutathione levels (p < 0.05). However, animals with hepatitis treated with FL-6 had a reduction of ALT activity (17.74%), as well as a reduction in IL-6 (24.21%) and IL-10 (30.91%) levels (p < 0.05). These animals showed a reduction in inflammatory response characterized by a decrease in inflammatory infiltrate at the hepatic parenchyma and portal structures; livers showed less damage and a reduction of necrotic and apoptotic hepatocytes. In conclusion, the treatment with FL-6 improved liver function and reduced the inflammatory marker in rats with chronic hepatitis induced by PS-administration.

免疫调节剂FL-6对Wistar大鼠慢性肝炎的药理学评价。
研究新型免疫调节药物FL-6对猪血清(PS)免疫诱导大鼠慢性肝炎的药理作用。32只雄性Wistar大鼠(150 g)分为4个实验组:(1)对照组(PBS 0.5 ml,每周3次,连续8周);(2) FL-6 (50 ng/kg,每周3次,连续4周);肝炎(PS 373 mg/kg,每周2次,持续8周);(4)肝炎+ FL-6(剂量如上)。治疗结束时处死大鼠。测定血清中ALT、AST、ALP、γ - gt活性及IL-6、IL-10水平。谷胱甘肽和丙二醛也进行了分析。对肝组织进行形态学分析。肝炎组ALT(1.44倍)、AST(1.28倍)、ALP(1.83倍)、γ - gt(3.91倍)、IL-6(2.6倍)、IL-10(7.1倍)水平较对照组升高(p < 0.05)。组织病理学分析显示炎症反应以炎症浸润和肝损伤为特征,伴有谷胱甘肽水平降低74.8% (p < 0.05)。然而,用FL-6治疗的肝炎动物ALT活性降低(17.74%),IL-6(24.21%)和IL-10(30.91%)水平降低(p < 0.05)。这些动物表现出炎症反应的减少,其特征是肝实质和门静脉结构的炎症浸润减少;肝脏损伤较小,坏死和凋亡肝细胞减少。综上所述,FL-6能改善ps诱导的慢性肝炎大鼠肝功能,降低炎症标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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