Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology.

Q2 Biochemistry, Genetics and Molecular Biology

Enzyme Research Pub Date : 2012-01-01 Epub Date: 2012-01-23 DOI:10.1155/2012/329178

Roberta Bussamara, Luciane Dall'agnol, Augusto Schrank, Kátia Flávia Fernandes, Marilene Henning Vainstein

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引用次数: 42

Abstract

This study aimed to develop an optimal continuous process for lipase immobilization in a bed reactor in order to investigate the possibility of large-scale production. An extracellular lipase of Pseudozyma hubeiensis (strain HB85A) was immobilized by adsorption onto a polystyrene-divinylbenzene support. Furthermore, response surface methodology (RSM) was employed to optimize enzyme immobilization and evaluate the optimum temperature and pH for free and immobilized enzyme. The optimal immobilization conditions observed were 150 min incubation time, pH 4.76, and an enzyme/support ratio of 1282 U/g support. Optimal activity temperature for free and immobilized enzyme was found to be 68°C and 52°C, respectively. Optimal activity pH for free and immobilized lipase was pH 4.6 and 6.0, respectively. Lipase immobilization resulted in improved enzyme stability in the presence of nonionic detergents, at high temperatures, at acidic and neutral pH, and at high concentrations of organic solvents such as 2-propanol, methanol, and acetone.

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响应面法连续固定化湖北假酵素(HB85A)脂肪酶的最佳条件研究

本研究旨在开发一种最佳的连续床反应器固定化脂肪酶的工艺，以探讨大规模生产的可能性。采用聚苯乙烯-二乙烯基苯载体吸附固定化湖北假酶HB85A胞外脂肪酶。在此基础上，采用响应面法(RSM)优化酶的固定化，并对游离酶和固定化酶的最佳温度和pH进行了评价。最佳固定化条件为:孵育时间150 min, pH为4.76，酶/载体比为1282 U/g载体。游离酶和固定化酶的最佳活性温度分别为68℃和52℃。游离脂肪酶和固定化脂肪酶的最佳活性pH分别为4.6和6.0。脂肪酶固定化导致酶在非离子洗涤剂、高温、酸性和中性pH、高浓度有机溶剂(如2-丙醇、甲醇和丙酮)下的稳定性得到改善。

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来源期刊

Enzyme Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

4.60

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0.00%

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