Effect of the 17beta-aminoestrogen pentolame on bone mineral levels in ovariectomized rats.

María Estela Avila, Sonia Angeles Garcia, Margarita Reyes Salas, Silvia Antuna, Cristina Lemini
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Abstract

Estrogens are fundamental to maintaining bone mineral balance. 17beta-aminoestrogens produce low estrogenic effects through ERalpha and ERbeta receptors, however their effects on bone tissue are unknown. This work evaluates the effects of the 17beta-aminoestrogen pentolame (AEP) and estradiol (E2) on the mineral profile of rat femur. Six months after ovariectomy (Ovx) adult Wistar rats (200-250g) were treated every third day for 30 days with subcutaneous (s.c.) injections of E2 (1, 10, 100 microg/kg), AEP (1, 10, 100, 500 microg/kg) or vehicle (propylenglycol; 1 ml/kg). After treatment, femur samples were prepared and Ca, P, Mg, Si, Fe, S, Na, K, and Cl concentration profiles were estimated using an X-ray analysis system coupled to an scanning electron microscope. Ovariectomy significantly decreased Ca, P, Mg and Si and increased Fe and S. Treatment with E2 restored Ca, P, Mg, and Si to the control values and decreased Fe and S in a dose dependent manner. AEP restored the levels of Ca, P, Mg and Si at all doses administered. AEP increased the levels of Fe and restored S to the basal level. The other minerals showed great variability and no significant differences were detected. Our results indicate differential action of AEP related to E2 in the restitution of bone mineral content.

17 -氨基雌激素对去卵巢大鼠骨矿物质水平的影响。
雌激素是维持骨矿物质平衡的基础。17 -氨基雌激素通过era和erb受体产生低雌激素效应,但其对骨组织的影响尚不清楚。本研究评价了17 - β -氨基雌激素、戊olame (AEP)和雌二醇(E2)对大鼠股骨矿物分布的影响。卵巢切除(Ovx) 6个月后,成年Wistar大鼠(200-250g)每隔3天皮下注射E2(1,10,100微克/千克),AEP(1,10,100, 500微克/千克)或对照物(丙二醇;1毫升/公斤)。处理后,制备股骨样品,并使用x射线分析系统耦合扫描电子显微镜估计Ca, P, Mg, Si, Fe, S, Na, K和Cl浓度谱。卵巢切除术显著降低Ca、P、Mg和Si,增加Fe和S。E2治疗使Ca、P、Mg和Si恢复到对照值,并以剂量依赖性方式降低Fe和S。AEP在所有剂量下都能恢复Ca、P、Mg和Si的水平。AEP提高了铁的水平,使S恢复到基础水平。其他矿物表现出很大的变异性,未发现显著差异。我们的结果表明AEP与E2在骨矿物质含量恢复中的差异作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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