Measuring and analyzing tissue specificity of human genes and protein complexes.

Dorothea Emig, Tim Kacprowski, Mario Albrecht
{"title":"Measuring and analyzing tissue specificity of human genes and protein complexes.","authors":"Dorothea Emig,&nbsp;Tim Kacprowski,&nbsp;Mario Albrecht","doi":"10.1186/1687-4153-2011-5","DOIUrl":null,"url":null,"abstract":"<p><p> Proteins and their interactions are essential for the survival of each human cell. Knowledge of their tissue occurrence is important for understanding biological processes. Therefore, we analyzed microarray and high-throughput RNA-sequencing data to identify tissue-specific and universally expressed genes. Gene expression data were used to investigate the presence of proteins, protein interactions and protein complexes in different tissues. Our comparison shows that the detection of tissue-specific genes and proteins strongly depends on the applied measurement technique. We found that microarrays are less sensitive for low expressed genes than high-throughput sequencing. Functional analyses based on microarray data are thus biased towards high expressed genes. This also means that previous biological findings based on microarrays might have to be re-examined using high-throughput sequencing results.</p>","PeriodicalId":72957,"journal":{"name":"EURASIP journal on bioinformatics & systems biology","volume":" ","pages":"5"},"PeriodicalIF":0.0000,"publicationDate":"2011-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1687-4153-2011-5","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"EURASIP journal on bioinformatics & systems biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/1687-4153-2011-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21

Abstract

Proteins and their interactions are essential for the survival of each human cell. Knowledge of their tissue occurrence is important for understanding biological processes. Therefore, we analyzed microarray and high-throughput RNA-sequencing data to identify tissue-specific and universally expressed genes. Gene expression data were used to investigate the presence of proteins, protein interactions and protein complexes in different tissues. Our comparison shows that the detection of tissue-specific genes and proteins strongly depends on the applied measurement technique. We found that microarrays are less sensitive for low expressed genes than high-throughput sequencing. Functional analyses based on microarray data are thus biased towards high expressed genes. This also means that previous biological findings based on microarrays might have to be re-examined using high-throughput sequencing results.

测量和分析人类基因和蛋白质复合物的组织特异性。
蛋白质及其相互作用对每个人类细胞的生存至关重要。了解它们的组织发生对于理解生物过程是很重要的。因此,我们分析了微阵列和高通量rna测序数据,以鉴定组织特异性和普遍表达的基因。基因表达数据用于研究蛋白质、蛋白质相互作用和蛋白质复合物在不同组织中的存在。我们的比较表明,组织特异性基因和蛋白质的检测在很大程度上取决于应用的测量技术。我们发现微阵列对低表达基因的敏感性低于高通量测序。因此,基于微阵列数据的功能分析偏向于高表达基因。这也意味着以前基于微阵列的生物学发现可能必须使用高通量测序结果重新检查。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信