A systems biology approach to analyse leaf carbohydrate metabolism in Arabidopsis thaliana.

Sebastian Henkel, Thomas Nägele, Imke Hörmiller, Thomas Sauter, Oliver Sawodny, Michael Ederer, Arnd G Heyer
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引用次数: 23

Abstract

Plant carbohydrate metabolism comprises numerous metabolite interconversions, some of which form cycles of metabolite degradation and re-synthesis and are thus referred to as futile cycles. In this study, we present a systems biology approach to analyse any possible regulatory principle that operates such futile cycles based on experimental data for sucrose (Scr) cycling in photosynthetically active leaves of the model plant Arabidopsis thaliana. Kinetic parameters of enzymatic steps in Scr cycling were identified by fitting model simulations to experimental data. A statistical analysis of the kinetic parameters and calculated flux rates allowed for estimation of the variability and supported the predictability of the model. A principal component analysis of the parameter results revealed the identifiability of the model parameters. We investigated the stability properties of Scr cycling and found that feedback inhibition of enzymes catalysing metabolite interconversions at different steps of the cycle have differential influence on stability. Applying this observation to futile cycling of Scr in leaf cells points to the enzyme hexokinase as an important regulator, while the step of Scr degradation by invertases appears subordinate.

Abstract Image

Abstract Image

Abstract Image

拟南芥叶片碳水化合物代谢的系统生物学分析方法。
植物碳水化合物代谢包括许多代谢物的相互转化,其中一些形成代谢物降解和再合成的循环,因此被称为无效循环。在这项研究中,我们提出了一种系统生物学方法来分析任何可能的调节原理,该原理基于模式植物拟南芥光合活性叶片中蔗糖(Scr)循环的实验数据。通过模型模拟与实验数据拟合,确定了Scr循环过程中酶促步骤的动力学参数。对动力学参数和计算出的通量率进行统计分析,可以对变率进行估计,并支持该模式的可预测性。参数结果的主成分分析揭示了模型参数的可辨识性。我们研究了Scr循环的稳定性,发现在循环的不同阶段,催化代谢物相互转化的酶的反馈抑制对稳定性有不同的影响。将这一观察结果应用于叶片细胞中Scr的无效循环,表明己糖激酶是一个重要的调节因子,而转化酶降解Scr的步骤似乎是次要的。
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