{"title":"Fractionation of volatile constituents from curcuma rhizome by preparative gas chromatography.","authors":"F Q Yang, H K Wang, H Chen, J D Chen, Z N Xia","doi":"10.1155/2011/942467","DOIUrl":null,"url":null,"abstract":"<p><p>A preparative gas chromatography (pGC) method was developed for the separation of volatile components from the methanol extract of Curcuma rhizome. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then, the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% were directed to the fraction collector. Five volatile compounds were collected from the methanol extract of Curcuma rhizome (5 g/mL) after 83 single injections (20 uL) with the yield of 5.1-46.2 mg. Furthermore, the structures of the obtained compounds were identified as β-elemene, curzerene, curzerenone, curcumenol, and curcumenone by MS and NMR spectra, respectively.</p>","PeriodicalId":15248,"journal":{"name":"Journal of Automated Methods & Management in Chemistry","volume":"2011 ","pages":"942467"},"PeriodicalIF":0.0000,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2011/942467","citationCount":"26","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Automated Methods & Management in Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2011/942467","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2011/8/18 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 26
Abstract
A preparative gas chromatography (pGC) method was developed for the separation of volatile components from the methanol extract of Curcuma rhizome. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then, the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the remaining 99% were directed to the fraction collector. Five volatile compounds were collected from the methanol extract of Curcuma rhizome (5 g/mL) after 83 single injections (20 uL) with the yield of 5.1-46.2 mg. Furthermore, the structures of the obtained compounds were identified as β-elemene, curzerene, curzerenone, curcumenol, and curcumenone by MS and NMR spectra, respectively.