A Novel Sandwich Electrochemical Immunosensor Based on the DNA-Derived Magnetic Nanochain Probes for Alpha-Fetoprotein.

Ning Gan, Liyong Jia, Lei Zheng
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引用次数: 11

Abstract

One novel electrochemical immunosensor was constructed by immobilizing capture antibody of alpha-fetoprotein (AFP Ab(1)) on a nafion/nanogold-particle modified glassy carbon electrode. With a sandwich immunoassay, one DNA-derived magnetic nanoprobe, simplified as DNA/(ZMPs-HRP-AFP Ab(2))(n), was employed for the detection of AFP. The fabricated procedure of the proposed biosensor was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The performance and factors influencing the performance of the biosensor were also evaluated. Under optimal conditions, the developed biosensor exhibited a well-defined electrochemical behavior toward the reduction of AFP ranging from 0.01 to 200 ng/mL with a detection limit of 4 pg/mL (S/N = 3). The biosensor was applied to the determination of AFP in serum with satisfactory results. It is important to note that the sandwich nanochainmodified electro-immunosensor provided an alternative substrate for the immobilization of other tumor markers.

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基于dna衍生磁性纳米链探针的新型夹心型甲胎蛋白电化学免疫传感器。
将甲胎蛋白捕获抗体(AFP Ab(1))固定在纳米金修饰的玻碳电极上,构建了一种新型的电化学免疫传感器。采用夹心免疫分析法,采用DNA来源的磁性纳米探针,简化为DNA/(ZMPs-HRP-AFP Ab(2))(n),检测AFP。利用循环伏安法和电化学阻抗谱对该传感器的制备过程进行了表征。并对生物传感器的性能及影响因素进行了评价。在最佳条件下,所研制的生物传感器在0.01 ~ 200 ng/mL范围内表现出良好的电化学行为,检测限为4 pg/mL (S/N = 3),可用于血清中AFP的检测,结果令人满意。值得注意的是,三明治纳米链修饰的电免疫传感器为其他肿瘤标记物的固定提供了一种替代底物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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